Human Anti-TREM2 Recombinant Antibody (clone 3)
CAT#: VS-0323-CJ60
This human anti-TREM2 antibody (clone 3) is a product that recognizes Human TREM2 ectodomain.
Gene Expression
Published Data
Normal Tissue
Figure 1 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_A_6_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_B_8_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10917/26581_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (hTCEpi, THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095970-TREM2
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ELISA
Figure 1 Validation of scFvs binding to TREM2 by ELISA.
Antigen TREM2 (a.a. 19-131) purified from insect cells (black bars) or a non-target protein (HisRS-ABD, grey bars) was added to a streptavidincoated plate, followed by the addition of scFvs (in bacteria supernatant).
SPR
Figure 2 Initial SPR Single Cycle Kinetic measurements of scFvs purified from E. coli using Biacore T200.
An anti-FLAG M2 antibody was immobilised on a CM5 sensor chip and FLAG-tagged scFv was captured on the surface. The same TREM2 antigen was injected with increasing concentrations (0.16, 0.8, 4, 20 and 100 nM). Response was measured in resonance units (RU) over time (s). Red lines represent the measured data values, and dashed black lines represent the fit of the curve based on a 1:1 Langmuir binding model.
IA
Figure 3 Luminex binding of scFvs against a panel of 27 proteins including TREM2.
Proteins were added to neutravidin coupled magnetic colour-coded beads before addition of scFv (bacteria supernatant). Binding was detected using an RPE-conjugated anti-FLAG M2 antibody and analysed using FLEXMAP 3D (Luminex corp.) recording median fluorescence intensity (MFI).
Inhib
Figure 4 Assay of scFv-3 dimer showing scFvconcentration-dependent reduction in shedding.
SPR
Figure 5 Kinetic measurements of three anti-TREM2 scFvs and estimation of apparent KD values.
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Specifications
- Immunogen
- A TREM2 antigen comprising the Ig domain of human TREM2 (His19-Asp131) biotinylated on a C-terminal Avi tag
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human TREM2 ectodomain
- Species Reactivity
- Human
- Clone
- 3
- Applications
- Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance, Immunoassay, Inhibition
- Conjugate
- Unconjugated
- Related Disease
- Alzheimer's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 1 ng/mL-4 ng/mL
Surface Plasmon Resonance: 4 nM-350 nM
Target
- Alternative Names
- PLOSL2; TREM-2; Trem2a; Trem2b; Trem2c
- Gene ID
- 54209
- UniProt ID
- Q9NZC2
- Cellular Localization
- Cell membrane; Secreted
- Post Translation Modifications
- Undergoes ectodomain shedding through proteolytic cleavage by ADAM10 and ADAM17 to produce a transmembrane segment, the TREM2 C-terminal fragment (TREM2-CTF), which is subsequently cleaved by gamma-secretase.
- Protein Refseq
- NP_001258750.1; NP_061838.1
- Function
- Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849).
Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356).
Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (By similarity).
Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018).
Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (PubMed:29794134).
Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (By similarity).
Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881).
Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (By similarity).
Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (By similarity).
It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (By similarity).
During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (By similarity).
Plays a role in microglial MTOR activation and metabolism (By similarity).
Regulates age-related changes in microglial numbers (PubMed:29752066).
Triggers activation of the immune responses in macrophages and dendritic cells (PubMed:10799849).
Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (By similarity).
In dendritic cells, it mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640).
Involved in the positive regulation of osteoclast differentiation (PubMed:12925681).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| HPAB-0154-CN-S(P) | Mouse Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-0154-CN-S(P)) | WB, ELISA, FC, IHC, IF, IP | Mouse scFv |
| HPAB-0155-CN-S(P) | Mouse Anti-TREM2 Recombinant Antibody; scFv Fragment (HPAB-0155-CN-S(P)) | WB, ELISA, FC, IHC, IF, IP | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| HPAB-3341LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3341LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3342LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3342LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3343LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3343LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3344LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3344LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| HPAB-3345LY | Human Anti-TREM2 Recombinant Antibody (HPAB-3345LY) | FC, WB, IP, ELISA, IHC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3341LY-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-3341LY-F(E)) | FC, WB, IP, ELISA, IHC | Humanized Fab |
| HPAB-3342LY-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-3342LY-F(E)) | FC, WB, IP, ELISA, IHC | Humanized Fab |
| HPAB-3343LY-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-3343LY-F(E)) | FC, WB, IP, ELISA, IHC | Humanized Fab |
| HPAB-3344LY-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-3344LY-F(E)) | FC, WB, IP, ELISA, IHC | Humanized Fab |
| HPAB-3345LY-F(E) | Human Anti-TREM2 Recombinant Antibody; Fab Fragment (HPAB-3345LY-F(E)) | FC, WB, IP, ELISA, IHC | Humanized Fab |
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