Mouse Anti-TTR Recombinant Antibody (clone 14G8) (CAT#: HPAB-0642LY)
This product is an antibody that recognizes TTR. The antibody 14G8 can be used for immunoassay techniques such as: WB, FC, ELISA.
Specific Inquiry
WB
Figure 1 Characterization of 14G8 mAb by Western blot analysis.
Native TTR (TTR) and pH4-TTR (F) were separated by non-denaturing SDS-PAGE and Western blotted with 14G8 mAb. pH4-TTR migrated as a 15 kDa monomer and a 32 kDa non-native dimer.
Higaki, J. N., Chakrabartty, A., Galant, N. J., Hadley, K. C., Hammerson, B., Nijjar, T., ... & Tam, S. J. (2016). Novel conformation-specific monoclonal antibodies against amyloidogenic forms of transthyretin. Amyloid, 23(2), 86-97.
FuncS
Figure 2 TTR mAb-dependent phagocytosis.
All four anti-misTTR antibodies increased uptake of aggregated pHrodo-ATTR V30M in a dose-dependent fashion as measured by pHrodo-positive THP-1 cells. In contrast, no uptake was observed in cells treated with an isotype control IgG and no fluorescence was detected in untreated cells (red circle).
Higaki, J. N., Chakrabartty, A., Galant, N. J., Hadley, K. C., Hammerson, B., Nijjar, T., ... & Tam, S. J. (2016). Novel conformation-specific monoclonal antibodies against amyloidogenic forms of transthyretin. Amyloid, 23(2), 86-97.
IHC
Figure 3 Staining of TTR by the 14G8 mis-TTR mAb.
14G8 did stain intracellular TTR present in normal pancreatic α-cells.
Higaki, J. N., Chakrabartty, A., Galant, N. J., Hadley, K. C., Hammerson, B., Nijjar, T., ... & Tam, S. J. (2016). Novel conformation-specific monoclonal antibodies against amyloidogenic forms of transthyretin. Amyloid, 23(2), 86-97.
Specifications
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TTR
- Species Reactivity
- Human
- Clone
- 14G8
- Applications
- WB, FuncS, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunohistochemistry and Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
- Alternative Names
- Transthyretin; Prealbumin, Amyloidosis Type I; PALB; ATTR; TBPA; Epididymis Luminal Protein 111; Thyroxine-Binding Prealbumin;
- Gene ID
- 7276
- UniProt ID
- P02766
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2498-sdAb | Recombinant Anti-human TTR VHH Single Domain Antibody | ELISA, Neut, ICC, FC | Llama VHH |
| HPAB-0483-YJ-VHH | Recombinant Camel Anti-TTR Single Domain Antibody (HPAB-0483-YJ-VHH) | ELISA | Camel VHH |
| HPAB-0484-YJ-VHH | Recombinant Camel Anti-TTR Single Domain Antibody (HPAB-0484-YJ-VHH) | ELISA | Camel VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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