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    Mouse Anti-VIM Recombinant Antibody (clone 9E7E7) (CAT#: VS7-0425-WR1117)

    This product is a mouse IgG1 antibody that binds to human VIM. The antibody 9E7E7 can be used for WB and IHC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line ASC TERT1
    Figure 1 IF staining of human cell line ASC TERT1
    (Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
    Figure 3 IF staining of human cell line U-251 MG
    Figure 3 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Endothelial cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    nuclear
    Glial cells
    Staining:

    Medium

    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/
    membranous

    Neuropil
    Staining:

    Medium

    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/
    membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Endothelial cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%

    Fibroblasts
    Staining:

    High

    Intensity: Strong

    Mucosal lymphoid cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    Cells in tubules
    Staining:

    Medium

    Intensity: Strong
    Quantity: <25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Pachytene spermatocytes
    Staining:

    High

    Intensity: Strong
    Quantity: 75%-25%
    Peritubular cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%

    Sertoli cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
    Figure 8 Breast
    Figure 8 Breast
    (Adipocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    nuclear

    Myoepithelial cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    Non-germinal center cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    (Cell lines ordered by descending RNA expression order.)
    * https://v21.proteinatlas.org/ENSG00000026025-VIM
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

    Specifications

    • Immunogen
    • Recombinant human VIM fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human VIM
    • Species Reactivity
    • Human
    • Clone
    • 9E7E7
    • Molecule Class
    • Type III Intermediate Filament Protein
    • Applications
    • WB, IHC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Cataract 30, multiple types (CTRCT30)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • VIM; vimentin; HEL113; CTRCT30; epididymis luminal protein 113
    • Long Name
    • Vimentin
    • Cellular Localization
    • Cell membrane; Cytoplasm; Cytoskeleton; Intermediate filament; Membrane; Nucleus
    • Post Translation Modifications
    • Acetylation; Glycoprotein; Isopeptide bond; Phosphoprotein; S-nitrosylation; Ubl conjugation
    • Function
    • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity).; Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "VIM"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Mouse Antibodies IgG Antibodies Chicken IgY Antibodies Rabbit Monoclonal Antibodies MHC Tetramers for Cancer Fab Antibodies ScFv Antibodies MHC Tetramers for Other Applications IgM Antibodies Antibody Magnetic Beads ScFv-Fc Chimeras IHC Kits & Antibodies
    CAT Product Name Application Type
    TAB-353CQ Anti-Human VIM Recombinant Antibody (HoAKs-1) ELISA
    TAB-354CQ Anti-Human VIM Recombinant Antibody (2F6-1) IF
    TAB-355CQ Anti-Human VIM Recombinant Antibody (3F9-1) IF
    TAB-353CQ-S(P) Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1) ELISA, WB
    TAB-354CQ-S(P) Anti-Human VIM Recombinant Antibody scFv Fragment (2F6-1) ELISA, WB

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