Anti-VIM Recombinant Antibody (VS3-FY2915) (CAT#: VS3-FY2915)
The anti-VIM antibody is a recombinant monoclonal antibody that is specifically created to bind human VIM protein. The calculated molecular weight of the protein is about 53.7 kDa. The monoclonal antibody can be used in the area of Cataract 30, Multiple Types and Cataract 30.
| Sub Cat | Host Animal | Species Reactivity | Applications | Application Notes | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS3-FY2943 | Mouse | Human, Mouse, Rat | WB, IHC, FC, ELISA |
Western Blot: 1/500-1/2000 Immunohistochemistry: 1/50-1/200 Flow cytometry: 1/50-1/200 ELISA: 1/1000-1/10000 |
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| VS3-FY2916 | Mouse | Human | WB, IHC, ICC, IP |
Immunoprecipitation: 1/20 Western Blot: 1/500-1/1000 Immunohistochemistry: 1/50-1/100 Immunocytochemistry: 1/50-1/200 |
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| VS3-FY2917 | Rabbit | Human, Mouse, Rat, Hamster | WB, IHC, ICC |
Western Blot: 1/500-1/1000 Immunohistochemistry: 1/50-1/100 Immunocytochemistry: 1/50-1/200 |
More Infomation
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Figure 5 Colon
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
(Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Figure 7 Testis
(Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
(Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Figure 8 Breast
(Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
(Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Figure 9 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
Specifications
- Type
- IgG
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 53.7 kDa.
- Related Disease
- Cataract 30, Multiple Types and Cataract 30
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Vimentin
- Gene ID
- 7431
- UniProt ID
- P08670
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm
Cytoplasm, cytoskeleton
Nucleus matrix
Cell membrane
- Post Translation Modifications
- O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. (P08670-VIME_HUMAN)
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. (P08670-VIME_HUMAN)
Glycosylation at Ser7, Thr33, and Ser34 (NX_P08670 [NX_P08670-1])
Ubiquitination at Lys97, Lys120, Lys129, Lys139, Lys143, Lys168, Lys188, Lys223, Lys236, Lys282, Lys294, Lys313, Lys334, Lys373, Lys439, and Lys445 (NX_P08670 [NX_P08670-1])
Modification sites at PhosphoSitePlus (P08670)
Glycosylation from GlyConnect VIME_HUMAN (2867)
Glycosylation from GlyGen (P08670) 27 sites, 2 O-linked glycans (27 sites)
- Function
- Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. VIME_HUMAN,P08670
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CQ | Anti-Human VIM Recombinant Antibody (HoAKs-1) | ELISA | |
| TAB-354CQ | Anti-Human VIM Recombinant Antibody (2F6-1) | IF | |
| TAB-355CQ | Anti-Human VIM Recombinant Antibody (3F9-1) | IF | |
| TAB-353CQ-F(E) | Anti-Human VIM Recombinant Antibody Fab Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-F(E) | Anti-Human VIM Recombinant Antibody Fab Fragment (2F6-1) | ELISA, WB |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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