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Anti-VIM Recombinant Antibody (VS3-FY2915) (CAT#: VS3-FY2915)

The anti-VIM antibody is a recombinant monoclonal antibody that is specifically created to bind human VIM protein. The calculated molecular weight of the protein is about 53.7 kDa. The monoclonal antibody can be used in the area of Cataract 30, Multiple Types and Cataract 30.

Sub Cat Host Animal Species Reactivity Applications Application Notes Size Conjugation Endotoxin Purity Fc Engineering  
VS3-FY2943 Mouse Human, Mouse, Rat WB, IHC, FC, ELISA Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
VS3-FY2916 Mouse Human WB, IHC, ICC, IP Immunoprecipitation: 1/20
Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
VS3-FY2917 Rabbit Human, Mouse, Rat, Hamster WB, IHC, ICC Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunocytochemistry: 1/50-1/200
More Infomation
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%

Fibroblasts
Staining:

High

Intensity: Strong

Mucosal lymphoid cells
Staining:

High

Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:

High

Intensity: Strong
Quantity:>75%

Sertoli cells
Staining:

High

Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:

High

Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Figure 8 Breast
Figure 8 Breast
(Adipocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

Myoepithelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

Specifications

  • Type
  • IgG
  • Conjugate
  • Unconjugated, FITC
  • MW
  • The molecular weight of the target protein is approximately 53.7 kDa.
  • Related Disease
  • Cataract 30, Multiple Types and Cataract 30

Product Property

  • Clonality
  • Monoclonal Antibody
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Vimentin
  • Sequence Similarities
  • Belongs to the intermediate filament family.
  • Cellular Localization
  • Cytoplasm
    Cytoplasm, cytoskeleton
    Nucleus matrix
    Cell membrane
  • Post Translation Modifications
  • O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. (P08670-VIME_HUMAN)
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. (P08670-VIME_HUMAN)
    Glycosylation at Ser7, Thr33, and Ser34 (NX_P08670 [NX_P08670-1])
    Ubiquitination at Lys97, Lys120, Lys129, Lys139, Lys143, Lys168, Lys188, Lys223, Lys236, Lys282, Lys294, Lys313, Lys334, Lys373, Lys439, and Lys445 (NX_P08670 [NX_P08670-1])
    Modification sites at PhosphoSitePlus (P08670)
    Glycosylation from GlyConnect VIME_HUMAN (2867)
    Glycosylation from GlyGen (P08670) 27 sites, 2 O-linked glycans (27 sites)
  • Function
  • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
    Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. VIME_HUMAN,P08670
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Recommended Products

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT Product Name Application Type
TAB-353CQ Anti-Human VIM Recombinant Antibody (HoAKs-1) ELISA
TAB-354CQ Anti-Human VIM Recombinant Antibody (2F6-1) IF
TAB-355CQ Anti-Human VIM Recombinant Antibody (3F9-1) IF
TAB-353CQ-F(E) Anti-Human VIM Recombinant Antibody Fab Fragment (HoAKs-1) ELISA, WB
TAB-354CQ-F(E) Anti-Human VIM Recombinant Antibody Fab Fragment (2F6-1) ELISA, WB

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