Anti-VIM Recombinant Antibody (VS3-FY2915)
CAT#: VS3-FY2915
The anti-VIM antibody is a recombinant monoclonal antibody that is specifically created to bind human VIM protein. The calculated molecular weight of the protein is about 53.7 kDa. The monoclonal antibody can be used in the area of Cataract 30, Multiple Types and Cataract 30.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Normal Tissue
Figure 8 Breast
Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
❮
❯
| Sub Cat | Host Animal | Species Reactivity | Applications | Application Notes | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS3-FY2943 | Mouse | Human, Mouse, Rat | WB, IHC, FC, ELISA |
Western Blot: 1/500-1/2000 Immunohistochemistry: 1/50-1/200 Flow cytometry: 1/50-1/200 ELISA: 1/1000-1/10000 |
||||||
| VS3-FY2916 | Mouse | Human | WB, IHC, ICC, IP |
Immunoprecipitation: 1/20 Western Blot: 1/500-1/1000 Immunohistochemistry: 1/50-1/100 Immunocytochemistry: 1/50-1/200 |
||||||
| VS3-FY2917 | Rabbit | Human, Mouse, Rat, Hamster | WB, IHC, ICC |
Western Blot: 1/500-1/1000 Immunohistochemistry: 1/50-1/100 Immunocytochemistry: 1/50-1/200 |
More Infomation
Specifications
- Type
- IgG
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 53.7 kDa.
- Related Disease
- Cataract 30, Multiple Types and Cataract 30
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Vimentin
- Gene ID
- 7431
- UniProt ID
- P08670
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm
Cytoplasm, cytoskeleton
Nucleus matrix
Cell membrane
- Post Translation Modifications
- O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. (P08670-VIME_HUMAN)
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. (P08670-VIME_HUMAN)
Glycosylation at Ser7, Thr33, and Ser34 (NX_P08670 [NX_P08670-1])
Ubiquitination at Lys97, Lys120, Lys129, Lys139, Lys143, Lys168, Lys188, Lys223, Lys236, Lys282, Lys294, Lys313, Lys334, Lys373, Lys439, and Lys445 (NX_P08670 [NX_P08670-1])
Modification sites at PhosphoSitePlus (P08670)
Glycosylation from GlyConnect VIME_HUMAN (2867)
Glycosylation from GlyGen (P08670) 27 sites, 2 O-linked glycans (27 sites)
- Function
- Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. VIME_HUMAN,P08670
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "VIM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CQ | Anti-Human VIM Recombinant Antibody (HoAKs-1) | ELISA | |
| TAB-354CQ | Anti-Human VIM Recombinant Antibody (2F6-1) | IF | |
| TAB-355CQ | Anti-Human VIM Recombinant Antibody (3F9-1) | IF | |
| TAB-353CQ-F(E) | Anti-Human VIM Recombinant Antibody Fab Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-F(E) | Anti-Human VIM Recombinant Antibody Fab Fragment (2F6-1) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0741MZ | Chicken Anti-VIM Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3838 | Rabbit Anti-VIM Recombinant Antibody (clone DS3838AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1583-CN | Mouse Anti-VIM Recombinant Antibody (clone A6-C1) | WB, IF, IHC, FC | Mouse IgG2b |
| MRO-1584-CN | Rabbit Anti-VIM Recombinant Antibody (clone CBACN-580) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1585-CN | Mouse Anti-VIM Recombinant Antibody (clone D4-B11) | WB, IF, IHC, FC | Mouse IgG1 |
| ZG-0499F | Mouse Anti-VIM Recombinant Antibody (ZG-0499F) | WB, IHC, ELISA | Mouse IgG |
| VS3-WK2087 | Rabbit Anti-VIM (pS39) Recombinant Antibody (clone R38-1F-8) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-F(E) | Human Anti-VIM Recombinant Antibody Fab Fragment (HPAB-2717LY-F(E)) | FC, WB, Block, ELISA | Humanized Fab |
| HPAB-0349-YJ-F(E) | Mouse Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0349-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse Fab |
| HPAB-0350-YJ-F(E) | Human Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0350-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-S(P) | Human Anti-VIM Recombinant Antibody scFv Fragment (HPAB-2717LY-S(P)) | FC, WB, Block, ELISA | Humanized scfv |
| HPAB-0349-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0349-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| HPAB-0350-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0350-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF038 | DRB1*04:01/Human cit vimentin (GVYAT[cit]SSAV[cit]L[cit]SSVPGVR) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1237 | Mouse Anti-VIM Recombinant Antibody (clone 280CT3.4.6) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY274 | AbPlus™ Anti-VIM Magnetic Beads (NKI-M9) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY193 | Mouse Anti-VIM (clone HoAKs-1) scFv-Fc Chimera | WB, IHC, FC | Mouse IgG1, scFv-Fc |
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