Mouse Anti-VIM Recombinant Antibody (ZG-0500F) (CAT#: ZG-0500F)

Name: Mouse Anti-VIM Recombinant Antibody (ZG-0500F)
SKU: ZG-0500F
Rating: 5 (1 reviews)

This product is a mouse antibody that recognizes VIM.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Mouse Anti-VIM Recombinant Antibody (ZG-0500F) in WB.

Figure 1 Mouse Anti-VIM Recombinant Antibody (ZG-0500F) in WB.

Western blot analysis was performed using a vimentin monoclonal antibody against truncated vimentin recombinant protein.

Subcellular Location

Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg

Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg

Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg

Normal Tissue

Figure 4 Cerebral cortex
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg

Figure 5 Colon
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%

Fibroblasts
Staining:

High

Intensity: Strong

Mucosal lymphoid cells
Staining:

High

Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg

Figure 6 Kidney
(Cells in glomeruli
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg

Figure 7 Testis
(Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:

High

Intensity: Strong
Quantity:>75%

Sertoli cells
Staining:

High

Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:

High

Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg

Figure 8 Breast
(Adipocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

Myoepithelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg

Figure 9 Lymph node
(Germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg

RNA Expression

Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM

Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant fragment of Vimentin expressed in E. coli.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human VIM

Species Reactivity
Human

Applications
Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay

Conjugate
NONE

Product Property

Format
Liquid

Concentration
1 mg/mL

Storage
Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000
ELISA: 1/10000

Target

Alternative Names
Vimentin; VIM; Vimentin

Gene ID
7431

UniProt ID
P08670

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application
MHC-LC1806	APC-DRB*01:01/Human VIM (SAVRLRSSVPGVR) MHC Tetramer	FCM
MHC-LC1812	APC-DRB1*04:01/Human VIM (GVYATRSSAVRLR) MHC Tetramer	FCM
MHC-LC4137	PE-A*02:01/Human VIM (ELNDRFANYI) MHC Tetramer	FCM
MHC-LC4138	PE-A*68:02/Human VIM (ELNDRFANYI) MHC Tetramer	FCM
MHC-LC4139	PE-A*02:03/Human VIM (ELNDRFANYI) MHC Tetramer	FCM

CAT	Product Name	Application	Type
MRO-2273-CN	Rabbit Anti-VIM Polyclonal Antibody (MRO-2273-CN)	WB, IF, IHC, FC	Rabbit IgG
HPAB-0349-YJ	Mouse Anti-VIM Recombinant Antibody (HPAB-0349-YJ)	ELISA, WB, IP, ICC, IHC	Mouse IgG
MOB-117LC	Mouse Anti-VIM Recombinant Antibody (clone RV202), FITC	WB, FC, IHC	Mouse IgG
VS3-CJ514	Mouse Anti-VIM Recombinant Antibody (VS3-CJ514)	WB, ICC, IF, IHC	Mouse IgG
VS3-CJ515	Rabbit Anti-VIM Recombinant Antibody (VS3-CJ515)	WB, ICC, IF, IHC, IP, FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-2717LY-F(E)	Human Anti-VIM Recombinant Antibody Fab Fragment (HPAB-2717LY-F(E))	FC, WB, Block, ELISA	Humanized Fab
HPAB-0349-YJ-F(E)	Mouse Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0349-YJ-F(E))	ELISA, WB, IP, ICC, IHC, FC, IF	Mouse Fab
HPAB-0350-YJ-F(E)	Human Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0350-YJ-F(E))	ELISA, WB, IP, ICC, IHC, FC, IF	Humanized Fab

CAT	Product Name	Application	Type
HPAB-2717LY-S(P)	Human Anti-VIM Recombinant Antibody scFv Fragment (HPAB-2717LY-S(P))	FC, WB, Block, ELISA	Humanized scfv
HPAB-0349-YJ-S(P)	Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0349-YJ-S(P))	ELISA, WB, IP, ICC, IHC, FC, IF	Mouse scFv
HPAB-0350-YJ-S(P)	Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0350-YJ-S(P))	ELISA, WB, IP, ICC, IHC, FC, IF	Mouse scFv