Human Anti-EPHA3 (clone KB004) scFv-Fc Chimera
CAT#: VS-0425-FY104
This fusion protein targets EPHA3 (Eph receptor A3), a receptor tyrosine kinase involved in cell migration, adhesion, and proliferation. By inhibiting EPHA3, it can modulate cellular processes and has potential applications in cancer therapy and other diseases involving abnormal cell migration.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69390/1819_G10_18_cr59f32f9e0f828_selected.jpg
Normal Tissue
Figure 2 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_B_9_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (AF22)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044524-EPHA3
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Specifications
- Immunogen
- LK63 cells as an immunogen.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human EPHA3
- Species Reactivity
- Human
- Clone
- KB004
- Applications
- ADCC
- Related Disease
- Hematologic Cancer and Glioma Susceptibility 1
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- EPH Receptor A3; Tyrosine-Protein Kinase Receptor ETK1; Eph-Like Tyrosine Kinase 1; EPH-Like Kinase 4; EC 2.7.10.1; TYRO4; HEK4; ETK1; ETK; EK4
- Gene ID
- 2042
- UniProt ID
- P29320
- Long Name
- EPH Receptor A3
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
- Cellular Localization
- Cell membrane, Secreted
- Post Translation Modifications
- Autophosphorylates upon activation by EFNA5.
Phosphorylation on Tyr-602 mediates interaction with NCK1.
Dephosphorylated by PTPN1.
Glycosylation at Asn232, Asn337, Asn391, Asn404, and Asn493
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 7 sites, 3 N-linked glycans (3 sites)
- Protein Refseq
- XP_005264772.1; XP_047303629.1; XP_054201630.1; XP_054201631.1; NP_001397707.1
- Function
- Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.
The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5.
Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration.
Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1.
Involved in the retinotectal mapping of neurons.
May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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