Mouse Anti-ACTB Recombinant Antibody (clone 8H10D10) (CAT#: VS3-XY25)
Clone 8H10D10 is a mouse antibody, which reacts with human ACTB protein. This antibody is suitable for ACTB detection by WB, IHC assays.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Figure 5 Colon
(Enterocytes - Microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
(Enterocytes - Microvilli Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
Specifications
- Host Species
- Mouse
- Specificity
- Human ACTB
- Species Reactivity
- Human
- Clone
- 8H10D10
- Applications
- Western Blot, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/1000) and Immunohistochemistry(1/25).
Target
- Alternative Names
- Actin Beta; PS1TP5-Binding Protein 1; Beta Cytoskeletal Actin; Actin, Cytoplasmic 1; Beta-Actin; PS1TP5BP1; BRWS1
- Gene ID
- 60
- UniProt ID
- P60709
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-055 | Recombinant Anti-human ACTB VHH Single Domain Antibody | WB, ELISA, IP, FC, FuncS | Llama VHH |
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