Mouse Anti-ATF2 Recombinant Antibody (clone 4F12) (CAT#: VS3-XY99)
Clone 4F12 is a mouse antibody, which reacts with human ATF2 protein. This antibody is suitable for ATF2 detection by ELISA, WB, IHC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
Figure 4 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
Figure 9 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
Figure 10 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
Specifications
- Immunogen
- Recombinant fragment of human ATF2 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ATF2
- Species Reactivity
- Human
- Clone
- 4F12
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Activating Transcription Factor 2; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP Response Element-Binding Protein CRE-BP1; CAMP-Dependent Transcription Factor ATF-2; Histone Acetyltransferase ATF2; CREB-2; CREB2; HB16; Activating Transcription Factor 2 Splice Variant ATF2-Var2
- Gene ID
- 1386
- UniProt ID
- P15336
Related Resources
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
MAPK Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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