Mouse Anti-GPC1 scFv-Fc Chimera (VS-0125-FY60)
CAT#: VS-0125-FY60
The anti-GPC1 scFv-Fc fusion protein is a recombinant protein based on a single-chain variable fragment (scFv), which includes a single-chain antibody against GPC1 and the Fc region of IgG2a. It serves as a valuable tool for studying GPC1 expression. GPC1 is a membrane-associated glycoprotein that is often overexpressed in various malignant tumors. Therefore, using anti-GPC1 scFv-Fc to detect GPC1 expression is crucial for assessing the intensity and localization of GPC1 levels within tumors, aiding in the identification of suitable targets for CAR-T cell therapy.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30571/1715_D1_13_cr57fd06304494c_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate positivity in luminal membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30571/ihc_selected.jpg
Normal Tissue
Figure 3 Skin 1
High expression in skin 1
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70449/169210_B_8_1_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30571/64142_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30571/64142_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30571/64142_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000063660-GPC1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a, scFv-Fc
- Species Reactivity
- Human
- Applications
- Flow Cytometry, Immunohistochemistry, Immunofluorescence
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The anti-GPC1 scFv-Fc fusion protein has the potential to selectively bind to GPC1, providing a means to assess its expression in various cells or tissues. Utilizing this fusion protein in techniques such as flow cytometry (FACS), fluorescence microscopy, and immunohistochemical staining allows for the precise detection of GPC1 expression. This capability may facilitate the evaluation of GPC1 expression levels in tumor cells, offering insights into its role in cancer biology.
Target
- Alternative Names
- Glypican 1; Glypican Proteoglycan 1; Glypican-1; Glypican
- Gene ID
- 2817
- UniProt ID
- P35052
- Sequence Similarities
- Belongs to the glypican family.
- Cellular Localization
- Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
Endosome
- Post Translation Modifications
- S-nitrosylated in a Cu(2+)-dependent manner.
N- and O-glycosylated.
This cell-associated glypican is further processed to give rise to a medium-released species.
Glycosylation at Asn79, Asn116, Ser486, Ser488, Ser490, Thr512, and Thr515
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect GPC1_HUMAN (1279)
Glycosylation from GlyGen 13 sites, 15 N-linked glycans (2 sites), 3 O-linked glycans (7 sites)
- Protein Refseq
- XP_047299917.1; NP_002072.2
- Function
- Cell surface proteoglycan that bears heparan sulfate.
Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity).
May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
See other products for "GPC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3467z | Mouse Anti-GPC1 Recombinant Antibody (clone 32H1) | WB, IP, IF | Mouse IgG1, κ |
| MRO-1903-CN | Rabbit Anti-GPC1 Polyclonal Antibody (MRO-1903-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0152-YJ | Mouse Anti-GPC1 Recombinant Antibody (HPAB-0152-YJ) | ELISA, WB | Mouse IgG1, κ |
| VS3-FY604 | Recombinant Rabbit Anti-GPC1 Antibody (clone R03-2E8) | WB, ICC, IF | Rabbit IgG |
| VS-0224-XY54 | Mouse Anti-GPC1 Recombinant Antibody (VS-0224-XY54) | ELISA, WB, IF, IP, IHC-P | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-033CN | Human Anti-GPC1 Recombinant Antibody (NS-033CN) | WB, IF | Human IgG1 |
| NS-033CN-F(E) | Human Anti-GPC1 Recombinant Antibody; Fab Fragment (NS-033CN-F(E)) | WB, IF | Human Fab |
| NS-033CN-S(P) | Human Anti-GPC1 Recombinant Antibody; scFv Fragment (NS-033CN-S(P)) | WB, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0152-YJ-S(P) | Mouse Anti-GPC1 Recombinant Antibody; scFv Fragment (HPAB-0152-YJ-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-0153-YJ-S(P) | Mouse Anti-GPC1 Recombinant Antibody; scFv Fragment (HPAB-0153-YJ-S(P)) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0152-YJ-F(E) | Mouse Anti-GPC1 Recombinant Antibody; Fab Fragment (HPAB-0152-YJ-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-0153-YJ-F(E) | Mouse Anti-GPC1 Recombinant Antibody; Fab Fragment (HPAB-0153-YJ-F(E)) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY22 | Human Anti-GPC1 (clone K090-01a002) scFv-Fc Chimera | ADC, ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC623 | Recombinant Anti-GPC1 Vesicular Antibody, EV Displayed (VS-0425-YC623) | ELISA, FC, Cell-uptake |
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