Mouse Anti-KLF4 Recombinant Antibody (clone 1E5) (CAT#: VS3-XY980)
Clone 1E5 is a mouse antibody, which reacts with human KLF4 protein. This antibody is suitable for KLF4 detection by ELISA, WB, IHC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Figure 2 High expression in esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
Specifications
- Immunogen
- Recombinant fragment of human KLF4 (a.a. 2-470) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KLF4
- Species Reactivity
- Human
- Clone
- 1E5
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Kruppel Like Factor 4; Epithelial Zinc Finger Protein EZF; Gut-Enriched Krueppel-Like Factor; Kruppel-Like Factor 4 (Gut); Gut Kruppel-Like Factor; GKLF; EZF; Endothelial Kruppel-Like Zinc Finger Protein; Krueppel-Like Factor 4
- Gene ID
- 9314
- UniProt ID
- O43474
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1026CT | Recombinant Mouse anti-Human KLF4 Monoclonal antibody (2F6) | ELISA, ICC/IF, IHC-P, WB | |
| MRO-1997-CN | Rabbit Anti-KLF4 Polyclonal Antibody (MRO-1997-CN) | WB, IHC, FC | Rabbit IgG |
| ZG-0786J | Rabbit Anti-KLF4 Recombinant Antibody (clone 3A1) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-CJ149 | Mouse Anti-KLF4 Recombinant Antibody (VS3-CJ149) | WB | Mouse IgG1, κ |
| VS3-XY3408 | Rabbit Anti-KLF4 Recombinant Antibody (clone R03-5H7) | WB, IHC-P, ICC, IF | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1956 | Hi-Affi™ Recombinant Rabbit Anti-KLF4 Monoclonal Antibody (DS1956AB) | ELISA, IHC-P | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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