What are the main applications of this antibody?

This antibody is mainly used in flow cytometry. Through the PE Cy5 label, it can effectively detect and analyze cell populations expressing PTPRC. For example, it can be used in research to distinguish different types of immune cells and monitor the expression level of PTPRC on the cell surface and its changes under specific stimuli.

What are the related diseases?

PTPRC (CD45) is expressed in a variety of hematological malignancies, such as Acute Myeloid Leukemia (AML), Myelodysplastic Syndromes (MDS), Lymphoma, and Multiple Myeloma (MM). Therefore, it has become a potential therapeutic target for these diseases. In addition, PTPRC is also involved in the development of autoimmune diseases.

The target of this antibody is PTPRC (Protein Tyrosine Phosphatase Receptor C), which is a surface glycoprotein widely expressed in hematopoietic and immune cells. PTPRC plays an important role in cell activation, proliferation, and differentiation by regulating signal transduction pathways.

Can it be used for other species?

This antibody is specifically for mouse PTPRC and is not usually recommended for other species unless it has been strictly verified for its applicability in other species.

What experimental techniques can it be used for?

This antibody is suitable for flow cytometry and Immunofluorescence (IF). Although other techniques are not explicitly listed, it may also be suitable for and Immunohistochemistry (IHC) under appropriate conditions.

Mouse Anti-PTPRC Recombinant Antibody, PE-Cy5 (clone 4A9.4)

CAT#: VS3-XY1330

Clone 4A9.4 is a mouse antibody, which reacts with PTPRC protein. This antibody is suitable for PTPRC detection by FC, IF assays.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/if_selected.jpg

Normal Tissue Figure 2 Appendix

Lymphoid tissue
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_3_2.jpg

Normal Tissue Figure 3 Lymph node

Germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_8_8.jpg

RNA Expression Figure 4 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, JURKAT, MOLT-4, THP-1, U-266/70, U-266/84, U-698, U-937)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081237-PTPRC

❮

❯

Specifications

Host Species
Mouse

Type
Mouse IgG2a

Specificity
PTPRC

Species Reactivity
Human

Clone
4A9.4

Applications
Flow Cytometry, Immunofluorescence

Conjugate
PE-Cy5

Product Property

Format
Liquid

Storage
Store at 4°C away from light. Do not freeze.

Target

Alternative Names
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105

Gene ID
5788

UniProt ID
P08575

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Breast cancer biomarkers at key points during disease progression

High Quality Detection

I've used the Mouse Anti PTPRC Recombinant Antibody, PE Cy5 (clone 4A9.4) in my flow cytometry experiments. It's been amazing. The antibody's high specificity towards PTPRC in mouse cells allows for very accurate identification of the target cells. The PE Cy5 label gives a strong and clear signal, making the data analysis much easier. It has significantly enhanced my research on mouse immune cell subsets, which is crucial for understanding the immune system.

Reliable in Research

This antibody has been a reliable tool in my study related to hematopoietic cells. The fact that it is recombinant gives me confidence in its consistency. It has always shown excellent performance in detecting PTPRC, which is widely expressed in the cells I'm interested in. The low cross reactivity is also a great advantage. It helps me focus on the specific PTPRC related events without interference from other proteins, which is essential for my research.

Good Signal to Noise Ratio

In my experiments, this antibody has provided a very good signal to noise ratio. The specific binding to PTPRC in mouse cells is strong, while the background signal is minimal. This is especially important in flow cytometry where a clear signal is crucial for accurate cell sorting and analysis. The clone 4A9.4 seems to be very effective in achieving this high quality result, which has been a great help in my research on mouse immune cell functions.

Essential for PTPRC Studies

For anyone studying PTPRC in mouse models, this antibody is a must have. Its ability to specifically target PTPRC with high affinity makes it an invaluable tool. In my research on PTPRC's role in hematopoietic cell development, this antibody has been crucial. It helps me to precisely monitor the expression and distribution of PTPRC, which is key to understanding the complex processes involved in cell development and differentiation.

Stable and Dependable

I'm very satisfied with the stability of the Mouse Anti PTPRC Recombinant Antibody, PE Cy5 (clone 4A9.4). It has remained active and effective even after some storage time. The proper storage conditions are easy to follow, and as long as they are maintained, the antibody performs well. This stability and dependability make it a great choice for long term research projects involving PTPRC in mouse cells, such as my study on the long term changes in immune cell populations.

Q&As

What are the main applications of this antibody?

A: This antibody is mainly used in flow cytometry. Through the PE Cy5 label, it can effectively detect and analyze cell populations expressing PTPRC. For example, it can be used in research to distinguish different types of immune cells and monitor the expression level of PTPRC on the cell surface and its changes under specific stimuli.
What are the related diseases?

A: PTPRC (CD45) is expressed in a variety of hematological malignancies, such as Acute Myeloid Leukemia (AML), Myelodysplastic Syndromes (MDS), Lymphoma, and Multiple Myeloma (MM). Therefore, it has become a potential therapeutic target for these diseases. In addition, PTPRC is also involved in the development of autoimmune diseases.
What is the target?

A: The target of this antibody is PTPRC (Protein Tyrosine Phosphatase Receptor C), which is a surface glycoprotein widely expressed in hematopoietic and immune cells. PTPRC plays an important role in cell activation, proliferation, and differentiation by regulating signal transduction pathways.
Can it be used for other species?

A: This antibody is specifically for mouse PTPRC and is not usually recommended for other species unless it has been strictly verified for its applicability in other species.
What experimental techniques can it be used for?

A: This antibody is suitable for flow cytometry and Immunofluorescence (IF). Although other techniques are not explicitly listed, it may also be suitable for and Immunohistochemistry (IHC) under appropriate conditions.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-XY1330.pdf

MSDS

MSDS - VS3-XY1330.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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