Mouse Anti-ROR2 Recombinant Antibody (clone 6F2D10)
CAT#: VS3-XY1377
Clone 6F2D10 is a mouse antibody, which reacts with human ROR2 protein. This antibody is suitable for ROR2 detection by ELISA, WB, FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows distinct cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/50700_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/50700_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/50700_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/50700_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/50700_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21868/50700_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, HHSteC, HSkMC, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169071-ROR2/subcellular
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Specifications
- Immunogen
- Recombinant fragment of human ROR2 (a.a. 59-155) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ROR2
- Species Reactivity
- Human
- Clone
- 6F2D10
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
- Alternative Names
- Receptor Tyrosine Kinase Like Orphan Receptor 2; NTRKR2; Tyrosine-Protein Kinase Transmembrane Receptor ROR2; Neurotrophic Tyrosine Kinase, Receptor-Related 2; Receptor Tyrosine Kinase-Like Orphan Receptor 2; Neurotrophic Tyrosine Kinase Receptor-Related 2; EC 2.7.10.1; BDB1; BDB
- Gene ID
- 4920
- UniProt ID
- Q01974
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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