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Mouse Anti-SOD1 Recombinant Antibody (clone 6F5) (CAT#: VS3-XY1451)

Clone 6F5 is a mouse antibody, which reacts with SOD1 protein. This antibody is suitable for SOD1 detection by ELISA, WB, FC, ICC assays.


See more Engineered Antibody products for SOD1
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human cerebral cortex
Figure 1 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8670/ihc_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/30_D2_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/29_D2_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/31_D2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_B_9_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_7_5.jpg
Figure 9 Testis
Figure 9 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_4_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1401/4148_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142168-SOD1

Specifications

  • Immunogen
  • Recombinant fragment of human SOD1 expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • SOD1
  • Species Reactivity
  • Human
  • Clone
  • 6F5
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry

Product Property

  • Format
  • Liquid
  • Preservative
  • 0.03% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).

Target

  • Alternative Names
  • Superoxide Dismutase 1; Superoxide Dismutase 1, Soluble; HSod1; Amyotrophic Lateral Sclerosis 1 (Adult); Epididymis Secretory Protein Li 44; Superoxide Dismutase, Cystolic; Superoxide Dismutase [Cu-Zn]; Cu/Zn Superoxide Dismutase

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Single-domain Antibody

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MD-ZBF-YC022 Camelid Anti-SOD1 Recombinant Single Domain Antibody (clone Nb3) WB, IP, IHC, ELISA Camelid VHH
MD-ZBF-YC023 Camelid Anti-SOD1 Recombinant Single Domain Antibody (clone Nb4) WB, IP, IHC, ELISA Camelid VHH

Recombinant Antibody

CAT Product Name Application Type
MOB-1320z Mouse Anti-SOD1 Recombinant Antibody (clone 22C6) ELISA, FC, ICC, IF, WB Mouse IgG1
MRO-1456-CN Recombinant Rabbit Anti-SOD1 Monoclonal Antibody (JF1005) WB, IF, IHC, FC Rabbit IgG
VS3-FY1370 Recombinant Rabbit Anti-SOD1 Antibody (clone R04-4C6) WB, IHC-Fr, IHC-P, ICC, IF Rabbit IgG
VS3-FY1371 Recombinant Rabbit Anti-SOD1 Antibody (clone R07-1G6) WB, IHC-P Rabbit IgG
VS3-FY1372 Recombinant Rabbit Anti-SOD1 Antibody (clone R08-5A5) WB, IHC-Fr, IHC-P, ICC, IF Rabbit IgG

Mouse Antibody

CAT Product Name Application Type
TAB-466CT Mouse Anti-SOD1 Recombinant Antibody (TAB-466CT) WB Mouse IgG
TAB-467CT Mouse Anti-SOD1 Recombinant Antibody (TAB-467CT) WB, IF Mouse IgG
TAB-468CT Mouse Anti-SOD1 Recombinant Antibody (TAB-468CT) ELISA, WB, BL, IHC Mouse IgG
TAB-466CT-F(E) Mouse Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-466CT-F(E)) ELISA, WB, BL, IHC Mouse Fab
TAB-467CT-F(E) Mouse Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-467CT-F(E)) ELISA, WB, BL, IHC Mouse Fab

Human Antibody

CAT Product Name Application Type
TAB-469CT-F(E) Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-469CT-F(E)) ELISA Human Fab
TAB-470CT-F(E) Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-470CT-F(E)) ELISA Human Fab
TAB-471CT-F(E) Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-471CT-F(E)) ELISA Human Fab
TAB-472CT-F(E) Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-472CT-F(E)) ELISA Human Fab
TAB-473CT-F(E) Human Anti-SOD1 Recombinant Antibody; Fab Fragment (TAB-473CT-F(E)) IF, ELISA Human Fab

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3369 Hi-Affi™ Recombinant Rabbit Anti-SOD1 Monoclonal Antibody (DS3369AB) ICC/IF, IHC-P, IP, WB IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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