Mouse Anti-STAT5B Recombinant Antibody (clone 5B3) (CAT#: VS3-XY1473)
Clone 5B3 is a mouse antibody, which reacts with human STAT5B protein. This antibody is suitable for STAT5B detection by ELISA, WB assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5418/1972_G8_29_cr5e16e010302bf_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5418/1972_G8_29_cr5e16e010302bf_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4298/155189_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4298/155189_A_6_6_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_B_8_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_9_3.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_9_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_7_5.jpg
Figure 7 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_6_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_7_8.jpg
(Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49883/120962_A_7_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173757-STAT5B/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173757-STAT5B/subcellular
Specifications
- Immunogen
- Recombinant fragment of human STAT5B expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human STAT5B
- Species Reactivity
- Human
- Clone
- 5B3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
Related Resources
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Throid Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
ErbB Signaling Pathway
MAPK Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3860z | Mouse Anti-STAT5B Recombinant Antibody (clone 42B11) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-1956CT | Recombinant Mouse anti-Human STAT5B Monoclonal antibody (EML1952) | WB | |
| MRO-1442-CN | Recombinant Rabbit Anti-STAT5B Monoclonal Antibody (CBACN-533) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-117R | Mouse Anti-STAT5B Recombinant Antibody (ZG-117R) | WB, ELISA | Mouse IgG |
| VS3-CJ1022 | Rabbit Anti-STAT5B Recombinant Antibody (VS3-CJ1022) | WB, ICC, IF, IHC, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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