Rabbit Anti-Neu (phospho Y877) Antibody
CAT#: VS-0126-XL578
High-affinity rabbit polyclonal antibody targeting phospho-Tyrosine 877 of Neu (ErbB2/HER2). Validated for WB, IHC, and IP, it marks the activated receptor state common in many oncogenic signaling pathways.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Normal Tissue
Figure 5 Breast
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Myoepithelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (OE19, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
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Specifications
- Host Species
- Rabbit
- Specificity
- ERBB2
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IHC-p; IF(paraffin section); IP; ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Purified from antiserum by specific adsorption to the epitope immunogen during the affinity chromatography process.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
IHC: 1:100-1:300
IP: 2-5 μg/mg lysate
ELISA: 1:10000
Not yet tested in other applications.
Target
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Related Diseases
Breast Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Endocrine Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| MHC-YF1014 | APC-DRB1*04:01/Human HER2 (KVPIKWMALESILRRRF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL579 | Rabbit Anti-Neu (phospho T686) Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL1138 | Rabbit Anti-HER2 (phospho Y877) Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL1808 | Anti-Neu (phospho Y1221/Y1222) Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL1809 | Rabbit Anti-Neu (phospho Y1248) Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
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| VS-0126-XL1852 | Rabbit Anti-Neu (phospho Y1112) Antibody | Phospho Antibodies for Cell Signaling Research |
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