Rabbit Anti-PAKγ (phospho S197) Antibody
CAT#: VS-0126-XL529
This rabbit polyclonal antibody specifically targets PAKγ phosphorylated at Serine 197. It is optimized for IHC and IF applications to detect activated kinase localization within paraffin-embedded tissue sections.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HAP1
Immunofluorescent staining of human cell line HAP1 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44791/1918_E6_28_cr5cb48efe99beb_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Not detected Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_7_5.jpg
Normal Tissue
Figure 4 Cerebellum
Purkinje cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_B_9_8.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60219/142917_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HAP1, HDLM-2, NTERA-2, SCLC-21H, SH-SY5Y, U-2197, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077264-PAK3
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Specifications
- Host Species
- Rabbit
- Specificity
- PAK3
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- IHC-p; IF(paraffin section); ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Generated from antiserum and purified via affinity chromatography using the epitope-specific immunogen as the capture ligand.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- IHC: 1:100-1:300
ELISA: 1:5000
Not yet tested in other applications.
Target
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Related Diseases
Bladder Cancer
Related Signaling Pathways
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PAK3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1537CT | Recombinant Mouse anti-Human PAK3 Monoclonal antibody (EML2324) | ICC, IF, WB |
| CAT | Product Name | Application | Type |
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| MOR-2584 | Hi-Affi™ Recombinant Rabbit Anti-PAK3 Monoclonal Antibody (DS2584AB) | WB, IP, IHC | IgG |
| CAT | Product Name | Application | Type |
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| VS3-WK72 | Mouse Anti-PAK3 Recombinant Antibody (clone F11-G8) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS3-FY1092 | Recombinant Rabbit Anti-PAK3 (phospho Ser144) Antibody (clone R04-2A4) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY364 | Mouse Anti-NHP PAK3 Recombinant Antibody (clone 4G8F11) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS7-0425-WR557 | Mouse Anti-PAK3 Recombinant Antibody (clone 4G8A5) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5166 | Anti-Mouse PAK3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL531 | Rabbit Anti-PAKγ (phospho S141) Antibody | Phospho Antibodies for Cell Signaling Research |
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