Rabbit Anti-Smad5 (phospho S463/S465) Monoclonal Antibody (RCB-P0921)
CAT#: VS-0126-XL42
This rabbit-derived monoclonal identifies dual-phosphorylated S463/S465 on Smad5, indicating BMP pathway activation. It provides high-resolution results for researchers conducting protein localization studies using WB, ICC, and IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human uterus, post-menopause
Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
Normal Tissue
Figure 7 Endometrium
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
❮
❯
Specifications
- Host Species
- Rabbit
- Specificity
- Smad5 (phospho S463/S465)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; ICC; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Obtained by utilizing a dedicated Protein A adsorption method to secure a pure antibody preparation.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0126-XL42. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "SMAD5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3217z | Mouse Anti-SMAD5 Recombinant Antibody (clone 42D5) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1798MZ | Recombinant Mouse Anti-Human SMAD5 Antibody | ICC, IF, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3324 | Hi-Affi™ Recombinant Rabbit Anti-SMAD5 Monoclonal Antibody (DS3324AB) | ICC, IF, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1406-CN | Recombinant Rabbit Anti-SMAD5 Monoclonal Antibody (CBACN-514) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2381-CN | Recombinant Rabbit Anti-SMAD5 (phosphorylated Ser463/465) Monoclonal Antibody (CBACN-666) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0568U | Rabbit Anti-Phospho-SMAD5 (S463+S465) Recombinant Antibody (clone 2G11) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1079 | CytoStream™ Rabbit Anti-SMAD5 Recombinant Antibody (VS13-YC1079) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2108 | Anti-SMAD5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6724 | Anti-Mouse SMAD5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6723 | Anti-Human SMAD5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL132 | Rabbit Anti-Smad5 (phospho S463/S465) Monoclonal Antibody (RCB-P0582) | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL194 | Rabbit Anti-Smad5 (phospho S463/S465) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: ELISA, SPR, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
