Recombinant Rabbit Anti-Rat AGER Capture Antibody (clone R45-9J-5)
CAT#: VS3-WK1912
This antibody is a rabbit recombinant antibody against rat AGER. It was developed by immunization of rabbit with recombinant rat AGER protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R45-9J-5 and R87-7G-5. The clone R45-9J-5 can be used as a capture antibody in ELISA analysis to identify rat AGER.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64436/1232_G12_3_selected.jpg
Normal Tissue
Figure 2 Lung
Alveolar cells type I
Staining: High
Intensity: Strong
Quantity:>75%
Alveolar cells type II
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69474/168057_A_2_4.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (HEK93, JURKAT, RPTEC TERT1, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204305-AGER
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Specifications
- Immunogen
- Recombinant rat AGER protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Rat AGER
- Species Reactivity
- Rat
- Clone
- R45-9J-5
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- RAGE
- Gene ID
- 81722
- UniProt ID
- Q63495
- Long Name
- Advanced glycosylation end product-specific receptor
- Cellular Localization
- Membrane
- Post Translation Modifications
- Phosphorylated on its cytoplasmic domain by PKCzeta/PRKCZ upon ligand binding.
Targeted by the ubiquitin E3 ligase subunit FBXO10 to mediate its ubiquitination and degradation.
- Function
- Cell surface pattern recognition receptor that senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-44 | Recombinant Anti-human AGER Antibody | WB, FC, FuncS | IgG |
| HPAB-0341-FY | Mouse Anti-AGER Recombinant Antibody (HPAB-0341-FY) | WB, IHC, ELISA, FC | Mouse IgG, κ |
| HPAB-1476-FY | Mouse Anti-AGER Recombinant Antibody (HPAB-1476-FY) | ELISA | Mouse IgG |
| HPAB-1477-FY | Mouse Anti-AGER Recombinant Antibody (HPAB-1477-FY) | ELISA | Mouse IgG |
| HPAB-1478-FY | Mouse Anti-AGER Recombinant Antibody (HPAB-1478-FY) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-44-F(E) | Recombinant Anti-human AGER Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| PSBZ-175 | Rabbit Anti-AGER Recombinant Antibody (clone H4); scFv Fragment | ELISA, Neut | Rabbit scFv |
| HPAB-0086-YC-F(E) | Mouse Anti-AGER Recombinant Antibody Fab Fragment (HPAB-0086-YC-F(E)) | ELISA, RIA, IHC, Neut | Mouse Fab |
| HPAB-N0016-YC-F(E) | Human Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-N0016-YC-F(E)) | ELISA | Humanized Fab |
| HPAB-0341-FY-F(E) | Mouse Anti-AGER Recombinant Antibody Fab Fragment (HPAB-0341-FY-F(E)) | WB, IHC, ELISA, FC | Mouse Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-067 | Recombinant Anti-human AGER VHH Single Domain Antibody | WB, ELISA, IP, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-44-S(P) | Recombinant Human Anti-human AGER Antibody scFv Fragment | ELISA, WB, Neut, FuncS | scFv |
| PFBZ-175 | Rabbit Anti-AGER Recombinant Antibody (clone H4); Fab Fragment | ELISA, Neut | Rabbit Fab |
| HPAB-1476-FY-F(E) | Mouse Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-1476-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-1477-FY-F(E) | Mouse Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-1477-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-1478-FY-F(E) | Mouse Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-1478-FY-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-293CT | Anti-Human RAGE Recombinant Antibody (XT-M4) | WB, ELISA, Neut, PK | Humanized antibody |
| TAB-293CT-S(P) | Anti-Human RAGE Recombinant Antibody scFv Fragment (XT-M4) | SPR, IF, FC, WB, ELISA, Neut | Humanized antibody |
| TAB-293CT-F(E) | Anti-Human RAGE Recombinant Antibody Fab Fragment (XT-M4) | WB, ELISA, Neut | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-294CT-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (TAB-294CT-S(P)) | WB, ELISA, Neut | Mouse scFv |
| TAB-295CT-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (TAB-295CT-S(P)) | WB, ELISA, Neut | Mouse scFv |
| TAB-296CT-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (TAB-296CT-S(P)) | ELISA | Mouse scFv |
| TAB-297CT-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (TAB-297CT-S(P)) | ELISA | Mouse scFv |
| TAB-298CT-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (TAB-298CT-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-016CQ | Mouse Anti-AGER Recombinant Antibody (NEUT-016CQ) | WB, Neut | Mouse IgG2 |
| NEUT-017CQ | Mouse Anti-AGER Recombinant Antibody (clone CBL155) | Neut, WB | Mouse IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-018CQ | Mouse Anti-AGER Recombinant Antibody (clone CBL401) | Block, WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0100 | Hi-Affi™ Rabbit Anti-AGER Recombinant Antibody (clone DS100AB) | WB | Rabbit IgG |
| MOR-4724 | Hi-Affi™ Rabbit Anti-AGER Recombinant Antibody (clone TH238DS) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY56 | Anti-AGER Immunohistochemistry Kit | IHC | |
| VS-0525-XY210 | Anti-Mouse Ager Immunohistochemistry Kit | IHC | |
| VS-0525-XY209 | Anti-Human AGER Immunohistochemistry Kit | IHC | |
| VS-0525-XY211 | Anti-Rat AGER Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC31 | CytoStream™ Rabbit Anti-Ager Polyclonal Antibody (VS13-YC31) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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