Anti-Human RAGE Recombinant Antibody scFv Fragment (XT-M4) (CAT#: TAB-293CT-S(P))
Recombinant monoclonal antibody specifically binds to RAGE and neutralizes RAGE-associated activities. This antibody can be potentially used for treating RAGE-related disease.
Specific Inquiry
SPR
-1.png)
Figure 1 3B4 has a high affinity to mouse RAGE while the control M4 does not bind mouse RAGE.
SPR sensorgrams of A. anti-RAGE scFv (3B4) and B. control scFv (M4) using the Langmuir 1:1 binding model.
Kim, H. Y., Wang, X., Kang, R., Tang, D., Boone, B. A., Zeh III, H. J., ... & Edwards, W. B. (2018). RAGE-specific single chain Fv for PET imaging of pancreatic cancer. PloS one, 13(3), e0192821.
IF
-2.png)
Figure 2 Confocal microscopic images of RAGE expressing Panc02 cells.
Live cells were incubated with 3B4-Cy5 and M4-Cy5. After fixation, the nucleus was counterstained with DAPI (Scale bar = 10 μm).
Kim, H. Y., Wang, X., Kang, R., Tang, D., Boone, B. A., Zeh III, H. J., ... & Edwards, W. B. (2018). RAGE-specific single chain Fv for PET imaging of pancreatic cancer. PloS one, 13(3), e0192821.
FC
-3.png)
Figure 3 Flow cytometric analysis with fixed and live Panc02 cells demonstrate antibody-binding.
Cells were stained with 3B4-Cy5 and M4-Cy5 (n ≥ 3, ± SEM, *** p < 0.005 to the untreated cells).
Kim, H. Y., Wang, X., Kang, R., Tang, D., Boone, B. A., Zeh III, H. J., ... & Edwards, W. B. (2018). RAGE-specific single chain Fv for PET imaging of pancreatic cancer. PloS one, 13(3), e0192821.
Specifications
- Immunogen
- Human RAGE-Fc
- Host Species
- Humanized
- Type
- Humanized antibody
- Specificity
- Human
- Species Reactivity
- Human, mouse
- Clone
- XT-M4
- Applications
- SPR, IF, FC, WB, ELISA, Neut
- Related Disease
- RAGE-related disease
Applications
- Application Notes
- The RAGE antibody has been reported in applications of Surface Plasmon Resonance, Immunofluorescence, Flow Cytometry, Western Blot, Enzyme-linked Immunosorbent Assay, Neutralization.
For Surface Plasmon Resonanc: A Biacore X100 was used for binding kinetic study of purified scFvs. To evaluate the binding affinity of scFvs, recombinant mouse RAGE Fc Chimera was immobilized on a CM5 sensor chip via an amine coupling (~2,000 RU). The carboxyl groups of the reference cell were activated and quenched with aminoethanol. The anti-RAGE-Mab, scFvs and conjugates were diluted in HBS-EP buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, and 0.005% surfactant P20, pH 7.4) and flowed over the surface for 180 s at a rate of 30 μL/min followed by dissociation for 600 seconds. After each sample injection, the surface was regenerated with consecutive injections of 5 μL of 0.3% SDS solution and 3.3 μL 50 mM NaOH. All sensorgrams were double referenced by subtracting the surface effect from the control flow cell and the buffer effect from the blank buffer. The calculated kinetic values ka, kd, and KD were obtained using Biacore X100 Evaluation Software assuming the Langmuir 1:1 binding model. The fitted curves are superimposed on the binding isotherms.
For Flow Cytometry: Panc02 cells were harvested by trypsinization and fixed with 4% PFA. 5 x 104 cells were used for each labeling condition (n ≥ 3). Cells were incubated with 3B4-Cy5 and M4-Cy5 (2 μg) in the incubation buffer (100 μL) for 30 min on ice. Anti-mouse RAGE Mab (1/100) and Alexa Fluor 488 conjugated anti-mouse antibody (1/200) were sequentially incubated with cells for 30 min on ice. After each incubation, cells were washed with cold PBS three times. The same labeling conditions were used for live cell staining. Flow cytometric analysis was performed on a FACS LSR Fortessa flow cytometer using FACSDiva software provided by the University of Pittsburgh Cancer Institute Flow and Imaging Cytometry core facility, and the data was analyzed using VenturiOne software.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone XT-M4"
See other products for "AGER"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-067 | Recombinant Anti-human AGER VHH Single Domain Antibody | WB, ELISA, IP, FC, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-44-F(E) | Recombinant Human Anti-human AGER Antibody Fab Fragment | ELISA, WB, Dot, FuncS | Fab |
| HPAB-0086-YC-F(E) | Mouse Anti-AGER Recombinant Antibody (clone 7F9); Fab Fragment | ELISA, RIA, IHC, Neut | Mouse Fab |
| HPAB-N0016-YC-F(E) | Human Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-N0016-YC-F(E)) | ELISA | Humanized Fab |
| HPAB-1476-FY-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (HPAB-1476-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-1477-FY-S(P) | Mouse Anti-AGER Recombinant Antibody; scFv Fragment (HPAB-1477-FY-S(P)) | ELISA | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-175 | Rabbit Anti-AGER Recombinant Antibody (clone H4) | ELISA, Neut | Rabbit IgG |
| HPAB-0086-YC | Mouse Anti-AGER Recombinant Antibody (clone 7F9) | ELISA, RIA, IHC, Neut | Mouse IgG |
| MRO-1317-CN | Rabbit Anti-AGER Recombinant Antibody (clone JF0975) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0341-FY | Mouse Anti-AGER Recombinant Antibody (clone 2A11) | WB, IHC, ELISA, FC | Mouse IgG, κ |
| HPAB-1476-FY | Mouse Anti-AGER Recombinant Antibody (HPAB-1476-FY) | ELISA | Mouse IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-175 | Rabbit Anti-AGER Recombinant Antibody (clone H4); Fab Fragment | ELISA, Neut | Rabbit Fab |
| HPAB-N0016-YC-S(P) | Human Anti-AGER Recombinant Antibody; scFv Fragment (HPAB-N0016-YC-S(P)) | ELISA | Human scFv |
| HPAB-1476-FY-F(E) | Mouse Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-1476-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-1477-FY-F(E) | Mouse Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-1477-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-1478-FY-F(E) | Mouse Anti-AGER Recombinant Antibody; Fab Fragment (HPAB-1478-FY-F(E)) | ELISA | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-293CT | Anti-Human RAGE Recombinant Antibody (XT-M4) | WB, ELISA, Neut, PK | Humanized antibody |
| TAB-293CT-F(E) | Anti-Human RAGE Recombinant Antibody Fab Fragment (XT-M4) | WB, ELISA, Neut | Humanized antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-294CT | Mouse Anti-AGER Recombinant Antibody (TAB-294CT) | WB, ELISA, Neut | Mouse IgG |
| TAB-295CT | Mouse Anti-AGER Recombinant Antibody (TAB-295CT) | WB, ELISA, Neut | Mouse IgG |
| TAB-296CT | Mouse Anti-AGER Recombinant Antibody (TAB-296CT) | ELISA | Mouse IgG |
| TAB-297CT | Mouse Anti-AGER Recombinant Antibody (TAB-297CT) | ELISA | Mouse IgG |
| TAB-298CT | Mouse Anti-AGER Recombinant Antibody (TAB-298CT) | ELISA | Mouse IgG |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-016CQ | Mouse Anti-AGER Recombinant Antibody (clone 8D11) | WB, Neut | Mouse IgG2 |
| NEUT-017CQ | Mouse Anti-AGER Recombinant Antibody (clone CBL155) | Neut, WB | Mouse IgG2 |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-018CQ | Mouse Anti-AGER Recombinant Antibody (clone CBL401) | Block, WB, ELISA | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0100 | Hi-Affi™ Rabbit Anti-AGER Recombinant Antibody (clone DS100AB) | WB | Rabbit IgG |
| MOR-4724 | Hi-Affi™ Rabbit Anti-AGER Recombinant Antibody (clone TH238DS) | WB, FC | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for TAB-293CT-S(P). Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.