Recombinant Rabbit Anti-CANX Antibody (clone R07-2B1)
CAT#: VS3-FY186
This product is a recombinant rabbit antibody that recognizes human CANX protein. It can detect a band of approximately 68 kDa (observedd molecular weight: 90 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9433/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_A_6_6.jpg
Normal Tissue
Figure 7 Skin
Cells in basal layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Endothelial cells Staining: High Intensity: Strong Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: High Intensity: Strong Quantity: 75%-25% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_B_8_1.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4738/11907_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (hTERT-HME1, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127022-CANX
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❯
WB
Figure 1. Recombinant Rabbit Anti-CANX Antibody (clone R07-2B1) in WB.
Western blot analysis of Calnexin in K562 lysates using Calnexin Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human Calnexin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CANX
- Species Reactivity
- Human
- Clone
- R07-2B1
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 68 kDa; Observed MW: 90 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Calnexin; Major Histocompatibility Complex Class I Antigen-Binding Protein P88; IP90; P90; CNX
- Gene ID
- 821
- UniProt ID
- P27824
- Sequence Similarities
- Belongs to the calreticulin family.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Phosphorylated at Ser-564 by MAPK3/ERK1. Phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).
Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.
Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.
- Protein Refseq
- NP_001019820.1; NP_001737.1
- Function
- Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CANX"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3830z | Mouse Anti-CANX Recombinant Antibody (clone 42H7) | WB, FC, ICC, IF, IHC | Mouse IgG2b, κ |
| ZG-0358F | Mouse Anti-CANX Recombinant Antibody (ZG-0358F) | WB, IF, ELISA | Mouse IgG |
| VS3-QX149 | Mouse Anti-CANX Recombinant Antibody (clone 3H4A7) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-CJ841 | Rabbit Anti-CANX Recombinant Antibody (VS3-CJ841) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS7-HM289 | Mouse Anti-CANX Recombinant Antibody (clone CBL094HM) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0456 | Hi-Affi™ Rabbit Anti-CANX Recombinant Antibody (clone DS456AB) | IHC-P, IP, WB | Rabbit IgG |
| MOR-0079-FY | Rabbit Anti-CANX Recombinant Antibody (clone AFY0050) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-013CN | Mouse Anti-CANX Recombinant Antibody (clone CGMCCNo.3240) | ELISA, WB, IHC, IP, IF | Mouse IgG2b |
| NS-013CN-F(E) | Mouse Anti-CANX Recombinant Antibody (clone CGMCCNo.3240); Fab Fragment | ELISA, WB, IHC, IP, IF | Mouse Fab |
| NS-013CN-S(P) | Mouse Anti-CANX Recombinant Antibody (clone CGMCCNo.3240); scFv Fragment | ELISA, WB, IHC, IP, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY40 | AbPlus™ Anti-CANX Magnetic Beads (CGMCCNo.3240) | IP, Protein Purification | |
| VS-0724-YC826 | AbPlus™ Anti-CANX Magnetic Beads (VS-0724-YC826) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY304 | Anti-CANX Immunohistochemistry Kit | IHC | |
| VS-0525-XY968 | Anti-Mouse CANX Immunohistochemistry Kit | IHC | |
| VS-0525-XY967 | Anti-Human CANX Immunohistochemistry Kit | IHC | |
| VS-0525-XY969 | Anti-Canine CANX Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC117 | CytoStream™ Rabbit Anti-CANX Recombinant Antibody (VS13-YC117) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC443 | Recombinant Anti-CANX Vesicular Antibody, EV Displayed (VS-0425-YC443) | ELISA, FC, Cell-uptake |
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