Recombinant Rabbit Anti-Ccnb1 Antibody (clone R08-9B7)
CAT#: VS3-FY213
This product is a recombinant rabbit antibody that recognizes human Ccnb1 protein. It can detect a band of approximately 48 kDa (observedd molecular weight: 55 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
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WB
Figure 1. Recombinant Rabbit Anti-Ccnb1 Antibody (clone R08-9B7) in WB.
Western blot analysis of Cyclin B1 in Jurkat lysates using Cyclin B1 antibody.
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Specifications
- Immunogen
- Recombinant protein of mouse Cyclin B1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Ccnb1
- Species Reactivity
- Human
- Clone
- R08-9B7
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 48 kDa; Observed MW: 55 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin B1; G2/Mitotic-Specific Cyclin-B1
- Gene ID
- 891
- UniProt ID
- P14635
- Sequence Similarities
- Belongs to the cyclin family. Cyclin AB subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
- Protein Refseq
- NP_114172.1
- Function
- Essential for the control of the cell cycle at the G2/M (mitosis) transition.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCNB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1647z | Mouse Anti-CCNB1 Recombinant Antibody (clone 28C1) | WB, IF, IHC, IP, FuncS | Mouse IgG1, κ |
| MOB-2074MZ | Mouse Anti-CCNB1 Recombinant Antibody (clone jmw-qipt5.2) | IF, IP, WB | Mouse IgG |
| ZG-0445F | Mouse Anti-CCNB1 Recombinant Antibody (ZG-0445F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0205J | Mouse Anti-CCNB1 Recombinant Antibody (clone 1A5) | IHC-P, IF | Mouse IgG |
| ZG-0206J | Mouse Anti-CCNB1 Recombinant Antibody (clone 5C1) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0514 | Hi-Affi™ Rabbit Anti-CCNB1 Recombinant Antibody (clone DS514AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4322 | PE-B*57:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4323 | PE-A*29:02/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4324 | PE-B*58:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4410 | PE-A*01:01/Human CCNB1 (CSEYVKDIY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY146 | Mouse Anti-NHP CCNB1 Recombinant Antibody (clone V152) | WB, IHC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY334 | Anti-CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1081 | Anti-Mouse CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1080 | Anti-Human CCNB1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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