Recombinant Mouse Anti-CCND1 Antibody (clone 1F7F5)
CAT#: VS3-FY218
This product is a recombinant mouse antibody that recognizes CCND1 protein. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1667_B12_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in a fraction of basal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27802/1607_B12_2_red_green.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_9_4.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24/70_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (U-266/70, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110092-CCND1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CCND1
- Clone
- 1F7F5
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95%
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- Cyclin D1; B-Cell Lymphoma 1 Protein; B-Cell CLL/Lymphoma 1; BCL-1 Oncogene; PRAD1 Oncogene; PRAD1; BCL1; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1)
- Gene ID
- 595
- UniProt ID
- P24385
- Sequence Similarities
- Belongs to the cyclin family. Cyclin D subfamily.
- Cellular Localization
- Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex (PubMed:10766840, PubMed:33854232, PubMed:33854235, PubMed:33854239).
It also plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex following DNA damage (PubMed:19412162).
Ubiquitinated at Lys-269 by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-286 phosphorylation (PubMed:33854232, PubMed:33854235, PubMed:33854239).
The DCX(AMBRA1) complex represents the major regulator of CCND1 stability during the G1/S transition (PubMed:33854232, PubMed:33854235, PubMed:33854239).
Also ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity).
Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31 (PubMed:19412162).
SCF-type ubiquitination is dependent on Thr-286 phosphorylation (PubMed:10766840, PubMed:19412162).
Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner (PubMed:21952639).
Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization (PubMed:19917254).
- Protein Refseq
- NP_444284.1
- Function
- Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCND1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G004R | Anti-Ccnd1 immunotoxin (IgG)-RTA | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-262CQ | Mouse Anti-CCND1 Recombinant Antibody (clone DCS-6) | IHC-Fr, WB, Block, IHC-P, IF, FC, IP | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
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| MOR-0515 | Hi-Affi™ Rabbit Anti-CCND1 Recombinant Antibody (clone DS515AB) | IHC | Rabbit IgG |
| MOR-4566 | Hi-Affi™ Rabbit Anti-CCND1 Recombinant Antibody (clone TH76DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1799-CN | Rabbit Anti-CCND1 Polyclonal Antibody (MRO-1799-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1800-CN | Rabbit Anti-CCND1 Polyclonal Antibody (MRO-1800-CN) | WB, IHC | Rabbit IgG |
| ZG-0319U | Rabbit Anti-CCND1 Recombinant Antibody (clone E16-l) | IHC-P | Rabbit IgG |
| ZG-0358U | Rabbit Anti-CCND1 Recombinant Antibody (clone E16-I) | IHC-P | Rabbit IgG |
| VS3-CJ807 | Mouse Anti-CCND1 Recombinant Antibody (VS3-CJ807) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY148 | Mouse Anti-NHP CCND1 Recombinant Antibody (clone DCS-6) | WB, IF | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1085 | Anti-CCND1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1086 | Anti-Mouse CCND1 Immunohistochemistry Kit | IHC | |
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