Recombinant Rabbit Anti-CD99 Antibody (clone R02-4H1)
CAT#: VS3-FY328
This product is a recombinant rabbit antibody that recognizes human CD99 protein. It can detect a band of approximately 19 kDa (observedd molecular weight: 30 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35304/83205_A_1_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/205_G10_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/1728_B_9_5.jpg
Normal Tissue
Figure 5 Pancreas
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_3_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20/186_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000002586-CD99
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IHC
Figure 1. Recombinant Rabbit Anti-CD99 Antibody (clone R02-4H1) in IHC.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using a CD99 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human CD99
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD99
- Species Reactivity
- Human
- Clone
- R02-4H1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 19 kDa; Observed MW: 30 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- CD99 Molecule (Xg Blood Group); CD99 Antigen; Antigen Identified By Monoclonal Antibodies 12E7, F21 And O13; T-Cell Surface Glycoprotein E2; E2 Antigen; MIC2X; MIC2Y; MIC2; Antigen Identified By Monoclonal 12E7, Y Homolog; MIC2 (Monoclonal Antibody 12E7)
- Gene ID
- 4267
- UniProt ID
- P14209
- Sequence Similarities
- Belongs to the CD99 family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Extensively O-glycosylated.
- Protein Refseq
- NP_001116370.1; NP_001308296.1; NP_001308297.1
- Function
- Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CD99"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1118z | Mouse Anti-CD99 Recombinant Antibody (clone 40G11) | WB, FC, IHC | Mouse IgG1 |
| VS3-CJ649 | Mouse Anti-CD99 Recombinant Antibody (clone ABT-CD99) | IHC-P | Mouse IgG |
| VS3-CJ650 | Mouse Anti-CD99 Recombinant Antibody (clone PT1849) | IHC-P | Mouse IgG |
| VS4-WK96 | Mouse Anti-CD99 Recombinant Antibody (VS4-WK96) | ELISA, IHC | Mouse IgG |
| VS3-FY1678 | Rabbit Anti-CD99 Recombinant Antibody (clone R06-8G3) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-400LC | Human Anti-CD99 Recombinant Antibody (TAB-400LC) | FC, ELISA | Chimeric (Mouse/Human) IgG1 |
| TAB-400LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-400LC-S(P)) | FC, ELISA | Human scFv |
| TAB-400LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-400LC-F(E)) | FC, ELISA | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-401LC | Human Anti-CD99 Recombinant Antibody (TAB-401LC) | FC, ELISA, ADCC | Human IgG1 |
| TAB-401LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-401LC-S(P)) | FC, ELISA | Human scFv |
| TAB-401LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-401LC-F(E)) | FC, ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-402LC | Human Anti-CD99 Recombinant Antibody (TAB-402LC) | ELISA, FC | Human IgG |
| TAB-402LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-402LC-S(P)) | ELISA, FC | Human scFv |
| TAB-402LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-402LC-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0595 | Hi-Affi™ Rabbit Anti-CD99 Recombinant Antibody (clone DS595AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY209 | Human Anti-CD99 scFv-Fc Chimera (VS-0325-FY209) | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC180 | CytoStream™ Rabbit Anti-CD99 Recombinant Antibody (VS13-YC180) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC162 | Recombinant Anti-CD99 Vesicular Antibody, EV Displayed (VS-0425-YC162) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1250 | Anti-CD99 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1251 | Anti-Rat CD99 Immunohistochemistry Kit | IHC |
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