Recombinant Mouse Anti-CTNNB1 Antibody (clone 9A4-10E6-7G5) (CAT#: VS3-FY424)

• Immunogen
• Synthesized peptide derived from human Beta-catenin

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human CTNNB1

• Species Reactivity
• Human

• Clone
• 9A4-10E6-7G5

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• PBS pH 7.3, 50% Glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry: 1/100-1/500

• Alternative Names
• Catenin Beta 1; Catenin (Cadherin-Associated Protein), Beta 1, 88kDa; CTNNB; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Catenin (Cadherin-Associated Protein), Beta 1; Catenin Beta-1

• Gene ID
• 1499

• UniProt ID
• P35222

• Sequence Similarities
• Belongs to the beta-catenin family.

• Cellular Localization
• Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Synapse

• Post Translation Modifications
• Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity).
  Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase (PubMed:10966653, PubMed:12051714, PubMed:12027456).
  Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33 (PubMed:12077367, PubMed:25169422).
  Phosphorylated by NEK2 (PubMed:18086858).
  EGF stimulates tyrosine phosphorylation (PubMed:10187801).
  Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:20307497).
  Phosphorylation on Ser-191 and Ser-246 by CDK5 (PubMed:17009320).
  Phosphorylation by CDK2 regulates insulin internalization (PubMed:21262353).
  Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity (PubMed:20026641).
  Phosphorylation by SRC at Tyr-333 promotes interaction with isoform M2 of PKM (PKM2); promoting transcription activation (PubMed:22056988).
  Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation (PubMed:12077367).
  Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (PubMed:11389839, PubMed:11389840, PubMed:20307497).
  Ubiquitinated and degraded following interaction with SOX9 (By similarity).
  S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions.
  O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1.
  Deacetylated at Lys-49 by SIRT1.
  Phosphorylated at Thr-556 by herpes virus 1/HHV-1 leading to CTNNB1 inhibition.

• Protein Refseq
• NP_001091679.1; NP_001091680.1; NP_001895.1

• Function
• Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).

Secondary Antibody

• 2ND-049CQ
• Goat Anti-Mouse IgG2b Antibody, R-PE

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Select a product category from the dropdown menu below to view related products.