Recombinant Rabbit Anti-FCGR2A Antibody (clone R09-5D1)
CAT#: VS3-FY540
This product is a recombinant rabbit antibody that recognizes FCGR2A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R09-5D1 is specific for human FCGR2A. The antigen is synthetic peptide of human cd32.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1926_F1_3_selected.jpg
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Specifications
- Immunogen
- Synthetic peptide of human CD32.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FCGR2A
- Species Reactivity
- Human
- Clone
- R09-5D1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 35 kDa; Observed MW: 35-42 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Fc Fragment Of IgG Receptor IIa; Fc Fragment Of IgG, Low Affinity IIa, Receptor (CD32); Immunoglobulin G Fc Receptor II; IgG Fc Receptor II-A; Fc-Gamma-RIIa; FcRII-A; FCGR2A1; CDw32; IGFR2; FCG2
- Gene ID
- 2212
- UniProt ID
- P12318
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
- Protein Refseq
- NP_001129691.1; NP_067674.2
- Function
- Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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