Recombinant Rabbit Anti-FCGR2A Antibody (clone R09-5D1) (CAT#: VS3-FY540)
This product is a recombinant rabbit antibody that recognizes FCGR2A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R09-5D1 is specific for human FCGR2A. The antigen is synthetic peptide of human cd32.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1926_F1_3_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1926_F1_3_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14730/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong positivity in endothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14730/ihc_selected.jpg
Figure 3 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1847_F1_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10776/1847_F1_2_blue_red_green.jpg
Figure 4 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10718/26353_B_4_4.jpg
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10718/26353_B_4_4.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEL, HMC-1, K-562, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143226-FCGR2A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143226-FCGR2A
Figure 5 RNA cell line category: Cell line enhanced (HEL, HMC-1, K-562, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143226-FCGR2A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143226-FCGR2A
Specifications
- Immunogen
- Synthetic peptide of human CD32.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FCGR2A
- Species Reactivity
- Human
- Clone
- R09-5D1
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 35 kDa; Observed MW: 35-42 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- Fc Fragment Of IgG Receptor IIa; Fc Fragment Of IgG, Low Affinity IIa, Receptor (CD32); Immunoglobulin G Fc Receptor II; IgG Fc Receptor II-A; Fc-Gamma-RIIa; FcRII-A; FCGR2A1; CDw32; IGFR2; FCG2
- Gene ID
- 2212
- UniProt ID
- P12318
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
- Protein Refseq
- NP_001129691.1; NP_067674.2
- Function
- Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-982 | Recombinant Mouse Anti-Human CD32 Antibody F(ab')2 Fragment, clone 7.3 | Block, FC, ELISA, IF, ELISPOT | F(ab')2 |
| DrMAB-983 | Mouse Anti-FCGR2A Recombinant Antibody (clone 7.3); Fab Fragment | FC | Mouse Fab |
| TAB-039WM-F(E) | Human Anti-FCGR2A Recombinant Antibody (clone MDE-8); Fab Fragment | FC, WB, ELISA, Inhib | Human Fab |
| HPAB-0223-YC-F(E) | Mouse Anti-FCGR2A Recombinant Antibody Fab Fragment (HPAB-0223-YC-F(E)) | ELISA, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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