Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6) (CAT#: VS3-FY561)

Name: Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6)
SKU: VS3-FY561
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes FOXO3. This antibody has been reported for use in Western Blot. The clone R06-2G6 is specific for human, mouse, rat FOXO3. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser253 of human foxo3a.

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Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6) in WB.

Figure 1. Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6) in WB.

Western Blot analysis of Phospho-FOXO3A (Ser253) in K562, C6 lysates using Phospho-FOXO3A (Ser253) Antibody.

Subcellular Location

Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63104/if_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/if_selected.jpg

Normal Tissue

Figure 3 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/ihc_selected.jpg

Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_B_7_5.jpg

Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_3.jpg

Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_8_4.jpg

Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_5.jpg

RNA Expression

Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118689-FOXO3

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser253 of human FOXO3A.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat FOXO3

Species Reactivity
Human, Mouse, Rat

Clone
R06-2G6

Applications
Western Blot

Conjugate
Unconjugated

MW
Calculated MW: 71 kDa; Observed MW: 82 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000

Target

Alternative Names
Forkhead Box O3; Forkhead In Rhabdomyosarcoma-Like 1; FKHRL1; FOXO3A; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box Protein O3

Gene ID
2309

UniProt ID
O43524

Cellular Localization
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Secondary Antibody

Isotype Control

Related Resources

Related Diseases
Related Signaling Pathways

Gastric Cancer

Hepatocellular Carcinoma

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "FOXO3"

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IgG Antibody Products Rabbit Monoclonal Antibody Products Cancer Stem Cell Biomarker Specific Antibody Products

CAT	Product Name	Application	Type
MOB-1068MZ	Recombinant Mouse Anti-Human FOXO3 Antibody	IHC-P	Mouse antibody
MRO-0602-CN	Rabbit Anti-FOXO3 Recombinant Antibody (clone SP0054)	WB, IF	Rabbit IgG
VS3-CJ151	Rabbit Anti-FOXO3 Recombinant Antibody (VS3-CJ151)	WB, IHC	Rabbit IgG
VS3-XY3361	Rabbit Anti-FOXO3 Recombinant Antibody (clone R09-3J1)	WB, ICC, IF	Rabbit IgG
VS3-WK2258	Rabbit Anti-FOXO3 (pS253) Recombinant Antibody (VS3-WK2258)	WB	Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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