Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6)
CAT#: VS3-FY561
This product is a recombinant rabbit antibody that recognizes FOXO3. This antibody has been reported for use in Western Blot. The clone R06-2G6 is specific for human, mouse, rat FOXO3. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser253 of human foxo3a.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63104/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118689-FOXO3
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WB
Figure 1. Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6) in WB.
Western Blot analysis of Phospho-FOXO3A (Ser253) in K562, C6 lysates using Phospho-FOXO3A (Ser253) Antibody.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser253 of human FOXO3A.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat FOXO3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-2G6
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 71 kDa; Observed MW: 82 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Forkhead Box O3; Forkhead In Rhabdomyosarcoma-Like 1; FKHRL1; FOXO3A; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box Protein O3
- Gene ID
- 2309
- UniProt ID
- O43524
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
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Related Diseases
Gastric Cancer
Hepatocellular Carcinoma
Related Signaling Pathways
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "FOXO3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1068MZ | Recombinant Mouse Anti-Human FOXO3 Antibody | IHC-P | Mouse antibody |
| MRO-1871-CN | Rabbit Anti-FOXO3 Polyclonal Antibody (MRO-1871-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-XY3361 | Rabbit Anti-FOXO3 Recombinant Antibody (clone R09-3J1) | WB, ICC, IF | Rabbit IgG |
| VS3-WK2120 | Rabbit Anti-FOXO3 (pS235) Recombinant Antibody (clone R08-7U1) | WB, IHC-P | Rabbit IgG |
| VS3-WK2258 | Rabbit Anti-FOXO3 (pS253) Recombinant Antibody (VS3-WK2258) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1344 | Hi-Affi™ Rabbit Anti-FOXO3 Recombinant Antibody (clone DS1344AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC40 | Rabbit Anti-FOXO3 Antibody (VS-0924-YC40) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2634 | Anti-FOXO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2635 | Anti-Mouse FOXO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2636 | Anti-Rat FOXO3 Immunohistochemistry Kit | IHC |
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