Recombinant Rabbit Anti-HGF Detection Antibody (clone R01-4Y4)
CAT#: VS3-WK1703
This antibody is a rabbit recombinant antibody against human HGF. It was developed by immunization of rabbit with recombinant human HGF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R01-6Q9 and R01-4Y4 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R01-4Y4 can be used in ELISA analysis to identify human HGF.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40360/92262_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced.
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000019991-HGF
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Specifications
- Immunogen
- Recombinant human HGF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HGF
- Species Reactivity
- Human
- Clone
- R01-4Y4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Hepatocyte Growth Factor; Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor); Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen; Hepatopoietin-A; Scatter Factor; HPTA; SF
- Gene ID
- 3082
- UniProt ID
- P14210
- Long Name
- Hepatocyte Growth Factor
- Sequence Similarities
- Belongs to the peptidase S1 family. Plasminogen subfamily.
- Cellular Localization
- Extracellular region
- Post Translation Modifications
- The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
- Protein Refseq
- NP_000592.3
- Function
- Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
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| TAB-075CT | Human Anti-HGF Recombinant Antibody (TAB-075CT) | ELISA, Neut | Humanized antibody |
| TAB-075CT-S(P) | Human Anti-HGF Recombinant Antibody; scFv Fragment (TAB-075CT-S(P)) | ELISA, Neut | Humanized scFv |
| TAB-075CT-F(E) | Human Anti-HGF Recombinant Antibody; Fab Fragment (TAB-075CT-F(E)) | ELISA, Neut | Humanized Fab |
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| HPAB-0652-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0652-CN-S(P)) | ELISA, Neut | Mouse scFv |
| HPAB-0653-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0653-CN-S(P)) | ELISA, Neut | Mouse scFv |
| HPAB-0654-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0654-CN-S(P)) | ELISA, Neut | Mouse scFv |
| HPAB-0655-CN-S(P) | Mouse Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-0655-CN-S(P)) | ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-130 | Afuco™ Anti-HGF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-130) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-749 | Afuco™ Anti-HGF ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-749) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0713-FY-S(P) | Mouse Anti-HGF Recombinant Antibody (clone AB012); Fab Fragment | ELISA | Mouse Fab |
| HPAB-J0117-YC-F(E) | Human Anti-HGF Recombinant Antibody Fab Fragment (HPAB-J0117-YC-F(E)) | ELISA, Neut, FuncS | Chimeric (mouse/human) Fab |
| HPAB-1856-FY-S(P) | Human Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-1856-FY-S(P)) | ELISA | Human scFv |
| HPAB-1857-FY-S(P) | Human Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-1857-FY-S(P)) | ELISA | Human scFv |
| HPAB-1858-FY-S(P) | Human Anti-HGF Recombinant Antibody; scFv Fragment (HPAB-1858-FY-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1015 | Anti-HGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3130 | Anti-Mouse HGF Immunohistochemistry Kit | IHC | |
| VS-0525-XY3129 | Anti-Human HGF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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