Recombinant Rabbit Anti-HSPD1 Antibody (clone R02-8D9)
CAT#: VS3-FY713
This product is a recombinant rabbit antibody that recognizes HSPD1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-8D9 is specific for mouse, rat HSPD1. The antigen is human hsp60 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
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WB
Figure 1. Recombinant Rabbit Anti-HSPD1 Antibody (clone R02-8D9) in WB.
Western Blot analysis of Hsp60 in HeLa lysates using Hsp60 antibody.
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Specifications
- Immunogen
- Human Hsp60 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse, Rat HSPD1
- Species Reactivity
- Mouse, Rat
- Clone
- R02-8D9
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 61 kDa; Observed MW: 61 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Heat Shock Protein Family D (Hsp60) Member 1; Heat Shock 60kDa Protein 1 (Chaperonin); Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; 0 KDa Chaperonin; Chaperonin 60; HuCHA60; HSP-60; HSP60; CPN60; Spastic Paraplegia 13 (Autosomal Dominant)
- Gene ID
- 3329
- UniProt ID
- P10809
- Sequence Similarities
- Belongs to the chaperonin (HSP60) family.
- Cellular Localization
- Mitochondrion
- Protein Refseq
- NP_002147.2; NP_955472.1
- Function
- Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376).
The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSPD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1035 | Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0119JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0120JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-110CT | Anti-Human HSP60 Recombinant Antibody (prozumab) | Inhibit, ELISA | Humanized antibody |
| TAB-110CT-S(P) | Anti-Human HSP60 Recombinant Antibody scFv Fragment (prozumab) | Inhibit, ELISA | Humanized antibody |
| TAB-110CT-F(E) | Anti-Human HSP60 Recombinant Antibody Fab Fragment (prozumab) | Inhibit, ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-018CT | Recombinant Mouse Anti-HSP60 Antibody | WB, IHC, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-920CQ | Mouse Anti-HSPD1 Recombinant Antibody (clone 4B9/89) | Block, ELISA, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1704 | Rabbit Anti-HSPD1 Recombinant Antibody (clone DS1704AB) | IHC-P, WB | Rabbit IgG |
| MOR-0077-FY | Rabbit Anti-HSPD1 Recombinant Antibody (clone AFY0048) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2077 | PE-A*02:01/Human HSPD1 (KLSDGVAVL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1428LC | Mouse Anti-HSPD1 Recombinant Antibody (clone 9C9) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1944-CN | Rabbit Anti-HSPD1 Polyclonal Antibody (MRO-1944-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1945-CN | Rabbit Anti-HSPD1 Polyclonal Antibody (MRO-1945-CN) | WB, IF, IHC | Rabbit IgG |
| MOB-0044F | Mouse Anti-HSPD1 Recombinant Antibody (MOB-0044F) | IHC-P, IF | Mouse IgG |
| MOB-0045F | Mouse Anti-HSPD1 Recombinant Antibody (MOB-0045F) | IHC-P, IF | Mouse IgG |
| VS3-CJ576 | Mouse Anti-HSPD1 Recombinant Antibody (VS3-CJ576) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF309 | Qa-1b/Human Hsp60 (GMKFDRGYI) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0119JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0120JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY134 | AbPlus™ Anti-HSPD1 Magnetic Beads (VS-0424-XY134) | IP, Protein Purification | |
| VS-0724-YC1195 | AbPlus™ Anti-Hspd1 Magnetic Beads (VS-0724-YC1195) | IP, Protein Purification | |
| VS-0724-YC1197 | AbPlus™ Anti-HSPD1 Magnetic Beads (VS-0724-YC1197) | IP, Protein Purification |
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