Recombinant Rabbit Anti-IL12A Capture Antibody (clone R08-4B4)
CAT#: VS3-WK1710
This antibody is a rabbit recombinant antibody against human IL12A. It was developed by immunization of rabbit with recombinant human IL12A protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R08-4B4 and R03-6R8. The clone R08-4B4 can be used as a capture antibody in ELISA analysis to identify human IL12A.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_A_8_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_A_4_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+ RasG12V, HDLM-2, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168811-IL12A
❮
❯
Specifications
- Immunogen
- Recombinant human IL12A protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL12A
- Species Reactivity
- Human
- Clone
- R08-4B4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Interleukin 12A
- Gene ID
- 3592
- UniProt ID
- P29459
- Long Name
- Interleukin 12A
- Sequence Similarities
- Belongs to the IL-6 superfamily.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_000873.2
- Function
- Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1394-VHH | Recombinant Anti-Human IL12A VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
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| MOB-0251MC | Rabbit Anti-IL12 Antibody | WB, ELISA | |
| MOB-015CQ | Mouse Anti-IL12A Recombinant Antibody (clone YC124) | ELISA, IHC | Mouse IgG |
| HPAB-1549-FY | Mouse Anti-IL12A Recombinant Antibody (HPAB-1549-FY) | Neut, WB, IHC | Mouse IgG |
| VS3-WK1525 | Rat Anti-IL12A Recombinant Antibody (clone 4AC17.8) | IF, FC | Rat IgG2a, κ |
| VS3-WK1711 | Recombinant Rabbit Anti-IL12A Detection Antibody (clone R03-6R8) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0280MZ | Chicken Anti-Interleukin-12A Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1160CQ | Rat Anti-Il12a Recombinant Antibody (clone C18.2) | ELISA, FuncS, Neut | Rat IgG2a, κ |
| NEUT-1165CQ | Mouse Anti-IL12A Recombinant Antibody (clone CBL732) | Neut, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1788 | Rabbit Anti-IL12A Recombinant Antibody (clone DS1788AB) | WB, IP | Rabbit IgG |
| MOR-4607 | Rabbit Anti-IL12A Recombinant Antibody (clone TH120DS) | WB | Rabbit IgG |
| MOR-4608 | Rabbit Anti-IL12A Recombinant Antibody (clone TH121DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1549-FY-F(E) | Mouse Anti-IL12A Recombinant Antibody; Fab Fragment (HPAB-1549-FY-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-1550-FY-F(E) | Mouse Anti-IL12A Recombinant Antibody; Fab Fragment (HPAB-1550-FY-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-1551-FY-F(E) | Mouse Anti-IL12A Recombinant Antibody; Fab Fragment (HPAB-1551-FY-F(E)) | WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1549-FY-S(P) | Mouse Anti-IL12A Recombinant Antibody; scFv Fragment (HPAB-1549-FY-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-1550-FY-S(P) | Mouse Anti-IL12A Recombinant Antibody; scFv Fragment (HPAB-1550-FY-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-1551-FY-S(P) | Mouse Anti-IL12A Recombinant Antibody; scFv Fragment (HPAB-1551-FY-S(P)) | WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY121 | Mouse Anti-IL12A (clone 5F2) scFv-Fc Chimera | Inhib | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3481 | Anti-IL12A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3482 | Anti-Mouse Il12a Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC163 | Recombinant Anti-IL12A Eliminating Antibody, pH-Sensitive (VS-0625-YC163) | Antigen-Sweeping In Vivo. | Mouse IgG |
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