Recombinant Rabbit Anti-IL12A Detection Antibody (clone R03-6R8)

Name: Recombinant Rabbit Anti-IL12A Detection Antibody (clone R03-6R8)
SKU: VS3-WK1711
Rating: 5 (1 reviews)

CAT#: VS3-WK1711

This antibody is a rabbit recombinant antibody against human IL12A. It was developed by immunization of rabbit with recombinant human IL12A protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R08-4B4 and R03-6R8 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R03-6R8 can be used in ELISA analysis to identify human IL12A.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_B_8_5.jpg

Normal Tissue Figure 2 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_A_8_3.jpg

Normal Tissue Figure 3 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_A_7_5.jpg

Normal Tissue Figure 4 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1886/7336_A_4_6.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+ RasG12V, HDLM-2, SK-BR-3)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168811-IL12A

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Specifications

Immunogen
Recombinant human IL12A protein

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human IL12A

Species Reactivity
Human

Clone
R03-6R8

Applications
ELISA

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Aggregation
< 5%

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
0.01M PBS, pH 7.2

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
Interleukin 12A

Gene ID
3592

UniProt ID
P29459

Long Name
Interleukin 12A

Sequence Similarities
Belongs to the IL-6 superfamily.

Cellular Localization
Secreted

Protein Refseq
NP_000873.2

Function
Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity.

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Related Diseases

Inflammatory Bowel Diseases

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-WK1711.pdf

MSDS

MSDS - VS3-WK1711.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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