Recombinant Rabbit Anti-IL18 Detection Antibody (clone R08-6N9) (CAT#: VS3-WK1725)
This antibody is a rabbit recombinant antibody against human IL18. It was developed by immunization of rabbit with recombinant human IL18 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R08-6S1 and R08-6N9 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R08-6N9 can be used in ELISA analysis to identify human IL18.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
Figure 4 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
Figure 6 Kidney
(Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
(Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
Specifications
- Immunogen
- Recombinant human IL18 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL18
- Species Reactivity
- Human
- Clone
- R08-6N9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Interleukin 18
- Gene ID
- 3606
- UniProt ID
- Q14116
- Long Name
- Interleukin 18
- Sequence Similarities
- Belongs to the IL-1 family.
- Cellular Localization
- Cytoplasm, Secreted
- Post Translation Modifications
- The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form.
- Protein Refseq
- NP_001230140.1
- Function
- Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses.
Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL18"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
Human Antibody Products
Chicken IgY Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
ScFv Antibody Products
Antibody Magnetic Beads
ScFv-Fc Chimera Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-081 | Mouse Anti-IL18 Recombinant Antibody (clone 125-2H); Fab Fragment | ELISA, Neut | Mouse Fab |
| HPAB-0065-YC-F(E) | Human Anti-IL18 Recombinant Antibody (clone LT28); Fab Fragment | ELISA, RIA, IHC, FuncS, Neut | Human Fab |
| FAMAB-0229-CN-F(E) | Human Anti-IL18 Recombinant Antibody (clone 1I1G); Fab Fragment | WB, ELISA | Human Fab |
| FAMAB-0041JF-F(E) | Human Anti-IL18 Recombinant Antibody (clone h18-108); Fab Fragment | IB, FC, ELISA, FuncS | Human Fab |
| HPAB-1546WJ-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (clone #125-2HmAb) | ELISA, WB | Human Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-WK1725. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
