Human Anti-IL18 Recombinant Antibody (clone h18-108) (CAT#: FAMAB-0041JF)

Recombinant human monoclonal antibody specially binds to human IL-18. This anti-IL-18 antibody h18-108 can be useful not only as epitope-defined antibodies to investigate the mechanism of IL-18 signaling, but also as therapeutic reagents for human disorders involving IL-18.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 The expression of IL-18 and IL-18R in the pig anterior pituitary gland.

Figure 1 The expression of IL-18 and IL-18R in the pig anterior pituitary gland.

The house-keeping gene, GAPDH, served as the internal standard.

Nagai, Y., Watanabe, K., Aso, H., Ohwada, S., Muneta, Y., & Yamaguchi, T. (2006). Cellular localization of IL-18 and IL-18 receptor in pig anterior pituitary gland. Domestic animal endocrinology, 30(2), 144-154.

IHC

Figure 2 Cellular localization of IL-18 in the pig anterior pituitary gland.

Figure 2 Cellular localization of IL-18 in the pig anterior pituitary gland.

Mirror sections shows the localization of IL-18 in somatotrophs.

Nagai, Y., Watanabe, K., Aso, H., Ohwada, S., Muneta, Y., & Yamaguchi, T. (2006). Cellular localization of IL-18 and IL-18 receptor in pig anterior pituitary gland. Domestic animal endocrinology, 30(2), 144-154.

IF

Figure 3 Double-immunofluorescence method for IL-18 and GH show somatotrophs with IL-18.

Figure 3 Double-immunofluorescence method for IL-18 and GH show somatotrophs with IL-18.

Colocalization of IL-18 and IL-18R in the somatotroph.

Nagai, Y., Watanabe, K., Aso, H., Ohwada, S., Muneta, Y., & Yamaguchi, T. (2006). Cellular localization of IL-18 and IL-18 receptor in pig anterior pituitary gland. Domestic animal endocrinology, 30(2), 144-154.

ELISA

Figure 4 Interference of IL-18 in the ELISA 1 of IL-18BPa.

Figure 4 Interference of IL-18 in the ELISA 1 of IL-18BPa.

Percent inhibition of the absorbance signal is shown.

Novick, D., Schwartsburd, B., Pinkus, R., Suissa, D., Belzer, I., Sthoeger, Z., ... & Dinarello, C. A. (2001). A novel IL-18BP ELISA shows elevated serum IL-18BP in sepsis and extensive decrease of free IL-18. Cytokine, 14(6), 334-342.


Specifications

  • Host Species
  • Human
  • Derivation
  • Human phage display scFv library
  • Type
  • Human IgG
  • Specificity
  • Human IL-18
  • Species Reactivity
  • Human
  • Clone
  • h18-108
  • Applications
  • WB, IF, IHC, ELISA
  • Related Disease
  • Autoimmune diseases, Allergies

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The IL18 antibody has been reported in applications of Western Blot, Immunofluorescence, Immunohistochemistry, Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • IGIF; IL-18; IL-1g; IL1F4
  • Function
  • Signaling
    blocking activity

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone h18-108"

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Chicken IgY Antibody

CAT Product Name Application Type
BRD-0290MZ Chicken Anti-Interleukin-18 Polyclonal IgY Indirect ELISA, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4248 Rabbit Anti-IL18 Recombinant Antibody (clone SI327DS) ELISA Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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