Recombinant Rabbit Anti-KRT18 Antibody (clone R04-1A7) (CAT#: VS3-FY821)
This product is a recombinant rabbit antibody that recognizes KRT18. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R04-1A7 is specific for human KRT18. The antigen is synthetic peptide of human cytokeratin 18.
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WB
Figure 1. Recombinant Rabbit Anti-KRT18 Antibody (clone R04-1A7) in WB.
Western Blot analysis of keratin 18 in HeLa lysates using a cytokeratin 18 antibody.
Specifications
- Immunogen
- Synthetic peptide of human cytokeratin 18.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KRT18
- Species Reactivity
- Human
- Clone
- R04-1A7
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 48 kDa; Observed MW: 48 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Keratin 18; Cell Proliferation-Inducing Gene 46 Protein; Keratin 18, Type I; CK-18; CYK18; K18
- Gene ID
- 3875
- UniProt ID
- P05783
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
- Protein Refseq
- NP_000215.1; NP_954657.1
- Function
- Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1264z | Mouse Anti-KRT18 Recombinant Antibody (clone 21C5) | FC, IF, IHC, WB | Mouse IgG1 |
| MRO-0441-CN | Recombinant Mouse Anti-KRT18 Monoclonal Antibody (6-19) | WB, IF, IHC | Mouse IgG2a |
| MRO-0442-CN | Recombinant Rabbit Anti-KRT18 Monoclonal Antibody (CBACN-180) | WB, IF, IHC, FC | Rabbit IgG |
| VS4-CJ6 | Recombinant Mouse Anti-KRT18 Antibody (VS4-CJ6) | ELISA, WB, IHC, IF | Mouse IgG |
| VS4-CJ7 | Recombinant Mouse Anti-KRT18 Antibody (VS4-CJ7) | ELISA, WB, ICC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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