Recombinant Rabbit Anti-MAPK14 (phospho Thr180) Antibody (clone R08-4E6)
CAT#: VS3-FY886
This product is a recombinant rabbit antibody that recognizes MAPK14. This antibody has been reported for use in Western Blot. The clone R08-4E6 is specific for human, mouse MAPK14. The antigen is a synthetic phosphopeptide corresponding to residues surrounding thr180 of human p38.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51825/834_A12_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_8_8.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40578/149032_B_5_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112062-MAPK14
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WB
Figure 1. Recombinant Rabbit Anti-MAPK14 (phospho Thr180) Antibody (clone R08-4E6) in WB.
Western Blot analysis of p38 (Phospho-T180/Y182) in 293, 3T3 lysates using Phospho-p38.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Thr180 of human p38.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse MAPK14
- Species Reactivity
- Human, Mouse
- Clone
- R08-4E6
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 41 kDa; Observed MW: 41 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 14; Mitogen-Activated Protein Kinase P38 Alpha; MAX-Interacting Protein 2; CSAID-Binding Protein; MAP Kinase P38 Alpha; P38 MAP Kinase; MAP Kinase 14; EC 2.7.11.24; SAPK2A; CSBP1; CSBP2; CSPB1; CSBP; Mxi2; Cytokine Suppressive Anti-Inflammatory Drug Binding Protein; Cytokine Suppressive Anti-Inflammatory Drug-Binding Protein
- Gene ID
- 1432
- UniProt ID
- Q16539
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAPK14"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0512MZ | Chicken Anti-RK Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2153 | Hi-Affi™ Recombinant Rabbit Anti-MAPK14 Monoclonal Antibody (DS2153AB) | ELISA, WB | IgG |
| MOR-4696 | Hi-Affi™ Recombinant Rabbit Anti-MAPK14 Monoclonal Antibody (TH210DS) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0963-CN | Recombinant Mouse Anti-MAPK14 Monoclonal Antibody (9-B9) | WB, IF, IHC | Mouse IgG2a |
| ZG-0013U | Rabbit Anti-MAPK14 Recombinant Antibody (clone 2C11) | ELISA, WB, IHC, IF | Rabbit IgG |
| ZG-0050U | Rabbit Anti-MAPK14 Recombinant Antibody (clone 6H12) | ELISA, WB | Rabbit IgG |
| ZG-0378U | Rabbit Anti-MAPK14 Recombinant Antibody (clone E17-S) | ELISA, WB, IP, ICC | Rabbit IgG |
| VS3-WK2261 | Rabbit Anti-MAPK14 (pT180) Recombinant Antibody (VS3-WK2261) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1292 | AbPlus™ Anti-MAPK14 Magnetic Beads (VS-0724-YC1292) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4233 | Anti-MAPK14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4234 | Anti-Mouse MAPK14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4235 | Anti-Rat MAPK14 Immunohistochemistry Kit | IHC |
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