Recombinant Rabbit Anti-P4HB Antibody (clone R07-3H3)
CAT#: VS3-FY1088
This product is a recombinant rabbit antibody that recognizes P4HB. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-3H3 is specific for human, mouse, rat P4HB. The antigen is synthetic peptide of human p4hb.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/if_selected.jpg
Normal Tissue
Figure 2 Small intestine
High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_2_val_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_6.jpg
Normal Tissue
Figure 4 Duodenum
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Glands of Brunner Staining: High Intensity: Strong Quantity:>75% Goblet cells Staining: High Intensity: Strong Quantity:>75% Paneth cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_9_2.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185624-P4HB
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WB
Figure 1. Recombinant Rabbit Anti-P4HB Antibody (clone R07-3H3) in WB.
Western Blot analysis of PDIA2 in K562, C6 lysates using P4HB antibody.
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Specifications
- Immunogen
- Synthetic peptide of human P4HB.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat P4HB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3H3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 57 kDa; Observed MW: 57 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Prolyl 4-Hydroxylase Subunit Beta; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide; Protein Disulfide Isomerase Family A, Member 1; Protein Disulfide Isomerase-Associated 1; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase, Beta Polypeptide; Collagen Prolyl 4-Hydroxylase Beta; EC 5.3.4.1; ERBA2L; PO4DB; PDIA1; P55; PDI; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase; Thyroid Hormone Binding Protein P55); Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase-Associated 1)
- Gene ID
- 5034
- UniProt ID
- P07237
- Sequence Similarities
- Belongs to the protein disulfide isomerase family.
- Cellular Localization
- Cell membrane, Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Phosphorylation of Ser-357 by FAM20C is induced by endoplasmic reticulum stress and results in a functional switch from oxidoreductase to molecular chaperone (PubMed:32149426).
It also promotes interaction with ERN1 (PubMed:32149426).
- Protein Refseq
- NP_000909.2
- Function
- This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins (PubMed:32149426).
At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "P4HB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1426z | Mouse Anti-P4HB Recombinant Antibody (clone 31F5) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-2363CT | Recombinant Mouse anti-Cow P4HB Monoclonal antibody (nBcdbn72940) | FC | |
| VS3-FY1086 | Recombinant Mouse Anti-P4HB Antibody (clone 3F12C11D2) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY1087 | Recombinant Mouse Anti-P4HB Antibody (clone 5D12A4E5) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY1089 | Recombinant Rabbit Anti-P4HB Antibody (clone R09-1F4) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1823CQ | Recombinant Mouse Anti-P4hb Antibody (RL77) | BL, ELISA, FC, GSA, ICC, IF, IHC, IHC-P, IP, WB | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1824CQ | Recombinant Mouse Anti-P4hb Antibody (RL90) | BL, ELISA, FC, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2568 | Hi-Affi™ Recombinant Rabbit Anti-P4HB Monoclonal Antibody (DS2568AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1441 | AbPlus™ Anti-P4HB Magnetic Beads (VS-0724-YC1441) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5140 | Anti-Mouse P4HB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5141 | Anti-Rabbit P4HB Immunohistochemistry Kit | IHC |
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