Recombinant Mouse Anti-POU2AF1 Antibody (VS4-CJ79)
CAT#: VS4-CJ79
This mouse anti-POU2AF1 antibody is a product that recognizes human POU2AF1. The immunogen of this antibody is a synthesized peptide derived from human POU2AF1. The mAb has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human tonsil
Immunohistochemical staining shows high expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11193/27220_A_6_8_selected.jpg
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Specifications
- Immunogen
- Synthesized peptide derived from human POU2AF1
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Human POU2AF1
- Species Reactivity
- Human
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).
Target
- Alternative Names
- BOB1; OBF1; OCAB; OBF-1
- Gene ID
- 5450
- UniProt ID
- Q16633
- Long Name
- POU class 2 homeobox associating factor 1
- Sequence Similarities
- Belongs to the POU2AF family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
- Protein Refseq
- NP_006226.2
- Function
- Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290).
It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176).
It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176).
It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290).
Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "POU2AF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2794 | Hi-Affi™ Recombinant Rabbit Anti-POU2AF1 Monoclonal Antibody (DS2794AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1934 | PE-B*51:01/Human POU2AF1 (DAYALNHTL) MHC Tetramer | FCM | |
| MHC-LC4449 | PE-B*15:01/Human POU2AF1 (LPHQPLATY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0054J | Mouse Anti-POU2AF1 Recombinant Antibody (ZG-0054J) | IHC-P, IF | Mouse IgG |
| VS3-QX894 | Mouse Anti-POU2AF1 Recombinant Antibody (clone 1170CT1.1.1) | WB | Mouse IgG1, κ |
| VS3-WK1013 | Mouse Anti-POU2AF1 Recombinant Antibody (VS3-WK1013) | IHC | Mouse IgG2a |
| VS3-WK1014 | Mouse Anti-POU2AF1 Recombinant Antibody (clone ABT-BOB1) | IHC-P | Mouse IgG2a, κ |
| VS3-FY1168 | Recombinant Mouse Anti-POU2AF1 Antibody (clone 4G9-4A8-3E8) | IHC-P | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC919 | CytoStream™ Rabbit Anti-POU2AF1 Recombinant Antibody (VS13-YC919) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5579 | Anti-POU2AF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5580 | Anti-Mouse POU2AF1 Immunohistochemistry Kit | IHC |
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