Recombinant Rabbit Anti-Mouse S100A4 Detection Antibody (clone R55-3Y-2)
CAT#: VS3-WK1925
This antibody is a rabbit recombinant antibody against mouse S100A4. It was developed by immunization of rabbit with recombinant mouse S100A4 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R82-5X-2 and R55-3Y-2 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R55-3Y-2 can be used in ELISA analysis to identify mouse S100A4.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
Normal Tissue
Figure 2 Lung.
High expression in lung
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
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Specifications
- Immunogen
- Recombinant mouse S100A4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse S100A4
- Species Reactivity
- Mouse
- Clone
- R55-3Y-2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- 42a; 18A2; Capl; FSp1; Mts1; pk9a; PeL98; metastasin
- Gene ID
- 20198
- UniProt ID
- P07091
- Long Name
- S100 calcium binding protein A4
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Protein Refseq
- NP_002952.1; NP_062427.1
- Function
- Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy.
Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
Isotype Control
- CAT
- Product Name
Secondary Antibody
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|---|---|---|---|
| MOB-3568z | Mouse Anti-S100A4 Recombinant Antibody (clone 24D3) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| TAB-378CT | Anti-Human S100A4 Recombinant Antibody (TAB-378CT) | FuncS, IHC, Neut, Inhib | Mouse IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-546CL | Human Anti-S100A4 Recombinant Antibody (TAB-546CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0135CL-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-0135CL-S(P)) | WB, ELISA, BI, Block, Inhib | Mouse scFv |
| TAB-0136CL-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-0136CL-S(P)) | WB, ELISA, BI, Block, Inhib | Mouse scFv |
| TAB-379CT-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-379CT-S(P)) | WB, IHC, Block | Mouse scFv |
| TAB-380CT-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-380CT-S(P)) | WB, IHC, Block | Mouse scFv |
| TAB-381CT-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-381CT-S(P)) | WB, IHC, Block | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-377CT | Anti-Human S100A4 Single Domain Antibody (TAB-377CT), Research Grade Biosimilar | WB, ELISA | Single domain antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3155 | Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1028 | CytoStream™ Rabbit Anti-S100A4 Recombinant Antibody (VS13-YC1028) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1992 | Anti-S100A4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6304 | Anti-Mouse S100A4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6303 | Anti-Human S100A4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC283 | Recombinant Anti-S100A4 Eliminating Antibody, pH-Sensitive (VS-0625-YC283) | Antigen-Sweeping In Vivo. | Mouse IgG |
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