Recombinant Mouse Anti-SGK1 Antibody (VS4-CJ113)
CAT#: VS4-CJ113
This mouse anti-SGK1 antibody is a product that recognizes human, mouse, rat SGK1. The immunogen of this antibody is synthetic peptide of SGK1 (350aa-430aa). The mAb has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51251/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25148/56215_A_2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22085/49011_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HHSteC, RPTEC TERT1, SK-MEL-30, TIME, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118515-SGK1
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Specifications
- Immunogen
- Synthetic Peptide of SGK1 (350aa-430aa)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat SGK1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- SGK
- Gene ID
- 6446
- UniProt ID
- O00141
- Long Name
- Serum/glucocorticoid regulated kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
- Cellular Localization
- Cytoplasm, Nucleus, Endoplasmic reticulum membrane, Cell membrane, Mitochondrion
- Post Translation Modifications
- Regulated by phosphorylation. Activated by phosphorylation on Ser-422 by mTORC2, transforming it into a substrate for PDPK1 which phosphorylates it on Thr-256. Phosphorylation on Ser-397 and Ser-401 are also essential for its activity. Phosphorylation on Ser-78 by MAPK7 is required for growth factor-induced cell cycle progression.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability.
- Protein Refseq
- NP_001137148.1; NP_001137149.1; NP_001137150.1
- Function
- Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na+ retention, renal K+ elimination, salt appetite, gastric acid secretion, intestinal Na+/H+ exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na+ channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K+ channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na+/K+ ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na+ transport than isoform 1.
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Related Signaling Pathways
mTOR Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| BRD-0530MZ | Chicken Anti-SGK (ab1) Polyclonal IgY | WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY6541 | Anti-Mouse SGK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6540 | Anti-Human SGK1 Immunohistochemistry Kit | IHC |
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