Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5) (CAT#: VS3-FY1511)

Name: Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5)
SKU: VS3-FY1511
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes UBE2C. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R08-4F5 is specific for human, mouse, rat UBE2C. The antigen is human ube2c recombinant protein.

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Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC-P

Figure 1. Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5) in IHC-P.

Figure 1. Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human tonsil using UBE2C antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg

Normal Tissue

Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg

Figure 4 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg

Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg

Figure 7 Tonsil
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg

Figure 8 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg

Figure 9 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg

Figure 10 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg

RNA Expression

Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human UBE2C recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat UBE2C

Species Reactivity
Human, Mouse, Rat

Clone
R08-4F5

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 20 kDa; Observed MW: 20 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Ubiquitin Conjugating Enzyme E2 C; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme C; E2 Ubiquitin-Conjugating Enzyme C; Ubiquitin-Protein Ligase C; UBCH10; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Cyclin-Selective Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2 C

Gene ID
11065

UniProt ID
O00762

Cellular Localization
Cytosol, Nucleoplasm, Nucleus, Plasma membrane

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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MOB-3665z	Mouse Anti-UBE2C Recombinant Antibody (clone 16G12)	WB, IHC, IF, ELISA	Mouse IgG1
FAMAB-1285CQ	Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41C-6)	Immuno-MRM, WB	Mouse IgG1
FAMAB-1286CQ	Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41A-1)	Immuno-MRM, WB, ELISA	Mouse IgG1
VS3-XY1575	Mouse Anti-UBE2C Recombinant Antibody (clone 1F5D3)	ELISA, WB, IHC	Mouse IgG2a
VS3-XY1576	Mouse Anti-UBE2C Recombinant Antibody (clone 4C10A7)	ELISA, WB, IHC	Mouse IgG2a

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