Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5)
CAT#: VS3-FY1511
This product is a recombinant rabbit antibody that recognizes UBE2C. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R08-4F5 is specific for human, mouse, rat UBE2C. The antigen is human ube2c recombinant protein.
Tested Data
Gene Expression
IHC-P
Figure 1. Recombinant Rabbit Anti-UBE2C Antibody (clone R08-4F5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using UBE2C antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
Normal Tissue
Figure 7 Tonsil
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
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Specifications
- Immunogen
- Human UBE2C recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat UBE2C
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4F5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 20 kDa; Observed MW: 20 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 C; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme C; E2 Ubiquitin-Conjugating Enzyme C; Ubiquitin-Protein Ligase C; UBCH10; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Cyclin-Selective Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2 C
- Gene ID
- 11065
- UniProt ID
- O00762
- Cellular Localization
- Cytosol, Nucleoplasm, Nucleus, Plasma membrane
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "UBE2C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3665z | Mouse Anti-UBE2C Recombinant Antibody (clone 16G12) | WB, IHC, IF, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1197MZ | Recombinant Mouse Anti-Human UBE2C Antibody | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3749 | Rabbit Anti-UBE2C Recombinant Antibody (clone DS3749AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0057 | APC-B*15:02/Human Ubiquitin-conjugating enzyme E2C (ELWKNPTAF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0058 | PE-B*15:02/Human Ubiquitin-conjugating enzyme E2C (ELWKNPTAF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1285CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41C-6) | Immuno-MRM, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1286CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41A-1) | Immuno-MRM, WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1211 | Mouse Anti-UBE2C Recombinant Antibody (clone 5D-3F) | WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY524 | Mouse Anti-NHP UBE2C Recombinant Antibody (clone 1F5D3) | WB, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2415 | Anti-UBE2C Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7691 | Anti-Rat UBE2C Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7690 | Anti-Human UBE2C Immunohistochemistry Kit | IHC |
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