Recombinant Anti-human CCR5 Intrabody [+36 GFP]

CAT#: IAB-B039(G)

Recombinant anti-CCR5 intrabody in fusion with +36GFP works within cells and is directed against human CCR5, expressed in 293T cells. CCR5-intrabody efficiently blocked surface expression of human CCR5 and thus prevented cellular interactions with CCR5-dependent HIV-1 and simian immunodeficiency virus envelope glycoprotein.

Gene Expression
Figure 1 RNA cell line category: Cell line enhanced (JURKAT, Karpas-707, U-266/84)

Specifications

  • Immunogen
  • human chemokine (C-C motif) receptor 5 (gene/pseudogene)
  • Host Species
  • rabbit
  • Type
  • scFv-(+36GFP)
  • Cell Penetrating Peptide
  • +36 GFP
    GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
  • Species Reactivity
  • human
  • Applications
  • Formats of immunological tests: Fluorescence-activated cell sorting; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional studies

Product Property

  • Purity
  • >97%, by SDS-PAGE under reducing conditions
  • Storage
  • 4 °C, -20°C if preferred

Target

  • Alternative Names
  • CCR5; chemokine (C-C motif) receptor 5 (gene/pseudogene); CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5; C-C chemokine receptor type 5; chemr13; HIV-1 fusion coreceptor; chemokine receptor CCR5; C-C motif chemokine receptor 5 A159A
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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