Mouse Anti-CD47 Recombinant Antibody (clone MIAP410)
CAT#: NEUT-388CQ
This antibody is a mouse monoclonal antibody that binds specifically to CD47 (IAP), and it can neutralize the bioactivity of CD47 (IAP).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/204_F3_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/205_F3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_4_6.jpg
Normal Tissue
Figure 7 Prostate
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_2_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Recognizes CD47 (IAP)
- Species Reactivity
- Human, Mouse, Rat
- Clone
- MIAP410
- Applications
- Block, IF
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD47; CD47 molecule; IAP; OA3; MER6; leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen; integrin-associated signal transducer)
- Gene ID
- 961
- UniProt ID
- Q08722
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Efficient
Using this antibody product has made our workflows more efficient. The high quality and reliability save us time and resources.
Research essential
This product has become essential in our research, providing the accuracy and reliability we need for our complex studies.
Great customer care
We received great customer care with prompt responses and helpful advice, making our experience with this product very positive.
Q&As
-
How long does an antibody last?
A: The shelf life of antibody products ranges from few months to many years, depending on formulation and storage circumstances.
-
How can antibodies be preserved?
A: To keep antibodies effective, store them according to the manufacturer's recommendations, usually in the refrigerator at a certain temperature.
-
Could I use the antibody by myself?
A: Antibodies should normally be utilized with the guidance of a healthcare practitioner or in accordance with special investigative protocols.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NEUT-388CQ, RRID: AB_3111569)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| TAB-279LC | Human Anti-CD47 Recombinant Antibody (TAB-279LC) | Block, FuncS | Human IgG, κ |
| TAB-278LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) | ELISA | Human scFv |
| TAB-279LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-279LC-S(P)) | ELISA | Human scFv |
| TAB-278LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-280LC | Human Anti-CD47 Recombinant Antibody (TAB-280LC) | ELISA | Humanized antibody |
| TAB-281LC | Human Anti-CD47 Recombinant Antibody (TAB-281LC) | ELISA | Humanized antibody |
| TAB-282LC | Human Anti-CD47 Recombinant Antibody (TAB-282LC) | ELISA | Humanized antibody |
| TAB-283LC | Human Anti-CD47 Recombinant Antibody (TAB-283LC) | ELISA | Humanized antibody |
| TAB-284LC | Human Anti-CD47 Recombinant Antibody (TAB-284LC) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
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| NEUT-387CQ | Mouse Anti-CD47 Recombinant Antibody (clone CBL377) | Block, FC, CyTOF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-389CQ | Rat Anti-CD47 Recombinant Antibody (clone miap301) | FC, FuncS, Neut, WB | Rat IgG2a, κ |
| CAT | Product Name | Application | Type |
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| MOR-0565 | Hi-Affi™ Rabbit Anti-CD47 Recombinant Antibody (clone DS565AB) | WB, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0616-CN | Recombinant Dromedary Anti-CD47 Single Domain Antibody (HPAB-0616-CN) | ELISA | VHH |
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| NBD-0017-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0017-YJ-VHH) | ELISA, SPR | Camelid VHH |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0331-YC | Mouse Anti-CD47 Recombinant Antibody (HPAB-N0331-YC) | ELISA, FC, Block | Mouse IgG1, κ |
| HPAB-N0332-YC | Mouse Anti-CD47 Recombinant Antibody (HPAB-N0332-YC) | ELISA, FC, Block | Mouse IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1082LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1082LY-S(P)) | ELISA | Human scfv |
| HPAB-1083LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1083LY-S(P)) | ELISA | Human scfv |
| HPAB-1084LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1084LY-S(P)) | ELISA | Human scfv |
| HPAB-1085LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1085LY-S(P)) | ELISA | Human scfv |
| HPAB-1086LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1086LY-S(P)) | ELISA | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1088LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1088LY-F(E)) | ELISA | Human Fab |
| HPAB-1089LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1089LY-F(E)) | ELISA | Human Fab |
| HPAB-1090LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1090LY-F(E)) | ELISA | Human Fab |
| HPAB-1091LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1091LY-F(E)) | ELISA | Human Fab |
| HPAB-1092LY-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-1092LY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0310-CN | Mouse Anti-CD47 Recombinant Antibody (clone 1A9) | IF, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY99 | Mouse Anti-NHP CD47 Recombinant Antibody (clone MEM-122) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY55 | CytoStream™ Mouse Anti-CD47 Recombinant Antibody (clone B6H12) | FC | Mouse IgG1, kappa |
| VS-0225-XY56 | CytoStream™ Mouse Anti-CD47 Recombinant Antibody (clone CC2C6) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY1191 | Anti-CD47 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1192 | Anti-Human CD47 Immunohistochemistry Kit | IHC |
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