Recombinant Alpaca Anti-CD47 Single Domain Antibody (HPAB-AP496-YC) (CAT#: HPAB-AP496-YC)
Provided are sdAb clones that antagonize the mouse CD47–SIRPα interaction. A4 was specific for mouse CD47, with minimal cross-reactivity toward human CD47 at concentrations up to 1 μM. Also, it was reported that A4, H5, and H9 blocked SIRPα tetramer binding to surface-disposed CD47.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/204_F3_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/204_F3_1_selected.jpg
Figure 2 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/205_F3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/205_F3_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_B_8_5.jpg
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_B_8_5.jpg
Figure 5 Colon
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_8_3.jpg
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_8_3.jpg
Figure 6 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_4_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_4_6.jpg
Figure 7 Prostate
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_2_5.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_2_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
Specifications
- Immunogen
- The extracellular Ig-like V-type domain (ECD) of mouse CD47, which is the sole extracellular domain that mediates interaction with SIRPα.
- Host Species
- Alpaca
- Type
- Alpaca VHH
- Specificity
- Mouse
- Species Reactivity
- Mouse
- Clone
- HPAB-AP496-YC
- Applications
- Flow Cytometry, Block, Function Assay
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-278LC | Human Anti-CD47 Recombinant Antibody (TAB-278LC) | Block, FuncS | Human IgG, κ |
| TAB-279LC | Human Anti-CD47 Recombinant Antibody (TAB-279LC) | Block, FuncS | Human IgG, κ |
| TAB-278LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) | ELISA | Human scFv |
| TAB-279LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-279LC-S(P)) | ELISA | Human scFv |
| TAB-278LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) | ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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