Anti-Mouse CSF1R Recombinant Antibody (AFS98) (CAT#: TAB-079MZ)
Recombinant monoclonal antibody to CSF-1R, AFS98, is a rat monoclonal antibody intended for the prophylaxis and treatment of related disease.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
FC
Figure 1 Representative dot plots and quantification of the F4/80+ Ly6C- and CSF1R+ of F4/80+ immune cell infiltrates of WT and Csf1–/– MC38 tumors derived from WT mice at day 21.
Graphs show mean ± SEM of data from at least 3 independent experiments or 5 to 10 individual mice per group.
Gyori, D., Lim, E. L., Grant, F. M., Spensberger, D., Roychoudhuri, R., Shuttleworth, S. J., ... & Hawkins, P. T. (2018). Compensation between CSF1R+ macrophages and Foxp3+ Treg cells drives resistance to tumor immunotherapy. JCI insight, 3(11).
Specifications
- Host Species
- Rat
- Specificity
- Mouse
- Clone
- AFS98
- Applications
- FC
Applications
- Application Notes
- The CSF1R antibody has been reported in applications of Flow Cytometry. TAMs were isolated from other leukocytes by positive selection for CD115 (anti-mouse CD115-PE, clone AFS98, Biolegend) used at a 1:200 dilution in FACS buffer and incubated at 4°C for 30 minutes. After washing, the cells were incubated with anti-PE microbeads (Miltenyi Biotec) at a 1:10 dilution in FACS buffer at 4°C for a further 20 minutes.
Target
- Alternative Names
- Fms; Fim2; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
- Gene ID
- 12978
- UniProt ID
- P09581
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-178CL | Afuco™ Anti-CSF1R Recombinant Antibody (AFC-178CL), ADCC Enhanced | ELISA, WB | Human IgG1 |
| AFC-TAB-H23 | Afuco™ Anti-CSF1R ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H23) | ELISA, IP, FC, FuncS, Neut | ADCC enhanced antibody |
| AFC-TAB-011ML | Afuco™ Anti-CSF1R ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-011ML) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
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