Human Anti-CSF1R Recombinant Antibody (clone RG7155)
CAT#: PABL-055
Recombinant Human Antibody (RG7155) is capable of binding to CSF1R, expressed in Chinese Hamster Ovary cells (CHO). This antibody could inhibit CSF-1 receptor (CSF-1R) activation.
Published Data
Gene Expression
Figure 1 Luminescence-based viability of M(IL-10), 6d differentiated under hypoxia (HPX) or normoxia (NMX) and inhibited for further 8d with 1 µg/mL RG7155 compared to IgG.
Pradel, L.P., Ooi, C.H., Romagnoli, S., et al. (2016). Macrophage susceptibility to emactuzumab (RG7155) treatment. Molecular Cancer Therapeutics, 15(12), 3077-3086.
Figure 2 Expression (log2-transformed RPKM) values of baseline and treated tumor biopsy samples from 31 patients enrolled in the Phase 1 Trial for CSF1-R and CD163.
Pradel, L.P., Ooi, C.H., Romagnoli, S., et al. (2016). Macrophage susceptibility to emactuzumab (RG7155) treatment. Molecular Cancer Therapeutics, 15(12), 3077-3086.
Figure 3 RG7155 inhibits monocyte survival during differentiation by TCM.
Monocytes were cultured for 6 days in TCM with 30 mg/ml RG7155 or huIgG, and survival was analyzed by DAPI staining. Columns depicted were normalized to viable cells in the corresponding huIgG control (at least three donors; mean ± SEM; pairwise t test; *p < 0.05, **p < 0.01). The corresponding M-CSF/GM-CSF ratio of TCM is indicated on the secondary scale (blue squares). TC-Macs were classified as GM-CSF/CSF-1 polarized and are depicted by black bars at a CSF-1/GM-CSF ratio <50.
Ries, C.H., Cannarile, M.A., Sabine Hoves, S., et al. (2014). Targeting Tumor-Associated Macrophages with Anti-CSF-1R Antibody Reveals a Strategy for Cancer Therapy. Cancer Cell, 25, 1-14.
Figure 4 Representative immunohistochemistry (IHC) images from one cynomolgus male animal is shown for each liver, colon, and lung from the vehicle-, 30 mg/kg RG7155-, and 100 mg/kg RG7155-treated groups (left).
Each group is represented by one male and one female treated once weekly for 2 weeks. Individual scores for the percent area coverage of the different cell types are shown for both animals per vehicle and dosing groups tested (right).
Ries, C.H., Cannarile, M.A., Sabine Hoves, S., et al. (2014). Targeting Tumor-Associated Macrophages with Anti-CSF-1R Antibody Reveals a Strategy for Cancer Therapy. Cancer Cell, 25, 1-14.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12323/201_C4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12323/29746_B_7_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8970/24179_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8970/24179_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8970/24179_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8970/24179_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (BEWO, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182578-CSF1R
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Specifications
- Immunogen
- Human colony stimulating factor 1 receptor
- Host Species
- Human
- Specificity
- Human CSF1R
- Species Reactivity
- Human
- Clone
- RG7155
- Applications
- FC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Functional Assay and Inhibition. For details, refer to published data.
Target
- Alternative Names
- CSF1R; colony stimulating factor 1 receptor; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R; macrophage colony-stimulating factor 1 receptor; CD115 antigen; CSF-1 receptor; FMS proto-oncogene; proto-oncogene c-Fms; macrophage colony stimulating factor I receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog
- Gene ID
- 1436
- UniProt ID
- A2VDG3
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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- CAT
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H23 | Anti-Human CSF1R Recombinant Antibody (TAB-H23) | WB, ELISA, IP, FC, FuncS, Neut, IF | IgG1 - kappa |
| TAB-076MZ | Human Anti-CSF1R Recombinant Antibody (TAB-076MZ) | WB | Human IgG4 |
| TAB-078MZ | Human Anti-CSF1R Recombinant Antibody (TAB-078MZ) | ELISA | Humanized IgG |
| TAB-076MZ-F(E) | Human Anti-CSF1R Recombinant Antibody; Fab Fragment (TAB-076MZ-F(E)) | WB | Humanized Fab |
| TAB-078MZ-F(E) | Human Anti-CSF1R Recombinant Antibody; Fab Fragment (TAB-078MZ-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-178CL | Afuco™ Anti-CSF1R Recombinant Antibody (AFC-178CL), ADCC Enhanced | ELISA, WB | Human IgG1 |
| AFC-TAB-H23 | Afuco™ Anti-CSF1R ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H23) | ELISA, IP, FC, FuncS, Neut | ADCC enhanced antibody |
| AFC-TAB-011ML | Afuco™ Anti-CSF1R ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-011ML) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-055 | Human Anti-CSF1R Recombinant Antibody (clone RG7155); scFv Fragment | FC, FuncS | Mouse scFv |
| HPAB-0288-FY-S(P) | Human Anti-CSF1R Recombinant Antibody; scFv Fragment (HPAB-0288-FY-S(P)) | ELISA | Human scFv |
| HPAB-0301-FY-S(P) | Human Anti-CSF1R Recombinant Antibody (clone huAb6); scFv Fragment | Block, ELISA | Humanized scFv |
| HPAB-0302-FY-S(P) | Human Anti-CSF1R Recombinant Antibody (clone 0311); scFv Fragment | ADCC, ELISA | Humanized scFv |
| HPAB-J0060-YC-S(P) | Human Anti-CSF1R Recombinant Antibody; scFv Fragment (HPAB-J0060-YC-S(P)) | ELISA, Block, ADCC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-077MZ | Mouse Anti-CSF1R Recombinant Antibody (TAB-077MZ) | ELISA | Mouse IgG2a, κ |
| TAB-079MZ | Anti-Mouse CSF1R Recombinant Antibody (AFS98) | FC | |
| TAB-077MZ-S(P) | Mouse Anti-CSF1R Recombinant Antibody; scFv Fragment (TAB-077MZ-S(P)) | ELISA | Mouse scFv |
| TAB-077MZ-F(E) | Mouse Anti-CSF1R Recombinant Antibody; Fab Fragment (TAB-077MZ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-508CQ | Mouse Anti-CSF1R Recombinant Antibody (clone CBL141) | Neut, WB | Mouse IgG1 |
| NEUT-509CQ | Mouse Anti-CSF1R Recombinant Antibody (clone CBL809) | WB, Neut | Mouse IgG1 |
| NEUT-510CQ | Mouse Anti-CSF1R Recombinant Antibody (clone MM0467-5V36) | WB, Neut | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0825 | Hi-Affi™ Rabbit Anti-CSF1R Recombinant Antibody (clone DS825AB) | ELISA | Rabbit IgG |
| MOR-4112 | Hi-Affi™ Rabbit Anti-CSF1R Recombinant Antibody (clone SI183DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0153-FY | Human Anti-CSF1R Recombinant Antibody (HPAB-0153-FY) | ELISA | Humanized IgG1 |
| HPAB-0177-FY | Human Anti-CSF1R Recombinant Antibody (HPAB-0177-FY) | Block | Human IgG |
| HPAB-AP311-YC | Mouse Anti-CSF1R Recombinant Antibody (HPAB-AP311-YC) | ELISA, Inhib | Mouse IgG |
| VS3-XY534 | Mouse Anti-CSF1R Recombinant Antibody (clone 10C3G5) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY535 | Mouse Anti-CSF1R Recombinant Antibody (clone 6B9B9) | ELISA, WB, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2085LY-F(E) | Human Anti-CSF1R Recombinant Antibody (clone 2F11-d8); Fab Fragment | ELISA | Human Fab |
| HPAB-2086LY-F(E) | Human Anti-CSF1R Recombinant Antibody (clone 2F11-f12); Fab Fragment | ELISA | Human Fab |
| HPAB-0963-FY-S(P) | Human Anti-CSF1R Recombinant Antibody; scFv Fragment (HPAB-0963-FY-S(P)) | WB, FC, ELISA | Human scFv |
| HPAB-0964-FY-S(P) | Human Anti-CSF1R Recombinant Antibody; scFv Fragment (HPAB-0964-FY-S(P)) | WB, FC, ELISA | Human scFv |
| HPAB-0965-FY-S(P) | Human Anti-CSF1R Recombinant Antibody; scFv Fragment (HPAB-0965-FY-S(P)) | WB, FC, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY97 | Human Anti-CSF1R (clone RG7155) scFv-Fc Chimera | FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC551 | Recombinant Anti-CSF1R Vesicular Antibody, EV Displayed (VS-0425-YC551) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1705 | Anti-CSF1R Immunohistochemistry Kit | IHC | |
| VS-0525-XY1706 | Anti-Mouse CSF1R Immunohistochemistry Kit | IHC |
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