Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC12) (CAT#: HPAB-0074-LSX)
Recombinant monoclonal antibody to CSPG4. Clone D2A-1H10-UC12 is a human monoclonal antibody that can be potentially used in the treatment of melanoma cancer.
Specific Inquiry
FC
Figure 1 Flow cytometry analysis of 14 unique D2A-1H10 mutant yeast clones reacting to 10 nM Nus-D2A-CSPG4 Antigen.
FACS histogram overlay of negative control (yeasts incubated with secondary antibodies without the antigen), parental yeast clone D2A-1H10, and 14 unique D2A-1H10 mutant yeast clones reacting to 10 nM Nus-D2A-CSPG4 antigen. The seven unique clones selected for further characterization are as follows: D2A-1H10-UC3, D2A-1H10-UC4, D2A-1H10-UC5, D2A-1H10-UC8, D2A-1H10-UC10, D2A-1H10-UC12, and D2A-1H10-UC16.
FC
Figure 2 Flow cytometry analysis of apparent affinity (KD) of D2A-1H10-UC8 and D2A-1H10-UC12 scFvs on H350 cells.
FACS binding of D2A-1H10-UC8 and D2A-1H10-UC12 scFvs at different concentrations to H350 cells was used for apparent affinity determination. Experiments were repeated at least three times and representative data are presented.
FC
Figure 3 Binding of mutant scFvs to H350 cell line was specific. No binding was seen against the negative control cell line HEK293.
Specifications
- Host Species
- Human
- Derivation
- Yeast display library
- Type
- Human IgG
- Specificity
- Human CSPG4
- Species Reactivity
- Human
- Clone
- D2A-1H10-UC12
- Applications
- ELISA, WB
- Related Disease
- Melanoma cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry.
Target
- Alternative Names
- Chondroitin Sulfate Proteoglycan 4; Melanoma-Associated Chondroitin Sulfate Proteoglycan 2; Chondroitin Sulfate Proteoglycan 4 (Melanoma-Associated); Melanoma Chondroitin Sulfate Proteoglycan; Chondroitin Sulfate Proteoglycan NG2; MCSP; MEL-CSPG; EC 2.7.8; EC 3.6.3; HMW-MAA; MCSPG; MSK16; NG2
- Gene ID
- 1464
- UniProt ID
- Q6UVK1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1806z | Mouse Anti-CSPG4 Recombinant Antibody (clone 43C3) | WB, FC, IHC | Mouse IgG1, κ |
| HPAB-0070-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0070-LSX) | ELISA | Mouse IgG1 |
| HPAB-0071-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0071-LSX) | ELISA | Mouse IgG1 |
| HPAB-0072-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0072-LSX) | ELISA | Mouse IgG1 |
| HPAB-0073-LSX | Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC8) | ELISA, WB | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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