Recombinant monoclonal antibody to CSPG4. Clone D2A-1H10-UC8 is a human monoclonal antibody that can be potentially used in the treatment of melanoma cancer.
Figure 1 Flow cytometry analysis of 14 unique D2A-1H10 mutant yeast clones reacting to 10 nM Nus-D2A-CSPG4 Antigen.
FACS histogram overlay of negative control (yeasts incubated with secondary antibodies without the antigen), parental yeast clone D2A-1H10, and 14 unique D2A-1H10 mutant yeast clones reacting to 10 nM Nus-D2A-CSPG4 antigen. The seven unique clones selected for further characterization are as follows: D2A-1H10-UC3, D2A-1H10-UC4, D2A-1H10-UC5, D2A-1H10-UC8, D2A-1H10-UC10, D2A-1H10-UC12, and D2A-1H10-UC16.
Figure 2 Flow cytometry analysis of apparent affinity (KD) of D2A-1H10-UC8 and D2A-1H10-UC12 scFvs on H350 cells.
FACS binding of D2A-1H10-UC8 and D2A-1H10-UC12 scFvs at different concentrations to H350 cells was used for apparent affinity determination. Experiments were repeated at least three times and representative data are presented.
Figure 3 Binding of mutant scFvs to H350 cell line was specific. No binding was seen against the negative control cell line HEK293.
Figure 4 Affinity (KD) of D2A-1H10-UC8 by BIAcore. Experiments were repeated at least three times and representative data are presented.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G013E | Anti-CSPG4 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G013S | Anti-CSPG4 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study | |
AGTO-G014E | Anti-CSPG4 immunotoxin (IgG)-PE | Cytotoxicity assay, Function study | |
AGTO-G014S | Anti-CSPG4 immunotoxin (IgG)-Sap | Cytotoxicity assay, Function study |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-102CT | Anti-Human CSPG4 Recombinant Antibody (D2A-1H10) | ELISA, WB | Human antibody |
TAB-102CT-S(P) | Anti-Human CSPG4 Recombinant Antibody scFv Fragment (D2A-1H10) | ELISA, WB | Human antibody |
TAB-102CT-F(E) | Anti-Human CSPG4 Recombinant Antibody Fab Fragment (D2A-1H10) | ELISA, WB | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-103CT-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (TAB-103CT-S(P)) | FC, ELISA, WB | Mouse scFv |
TAB-104CT-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (TAB-104CT-S(P)) | FC | Mouse scFv |
TAB-103CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-103CT-F(E)) | FC, ELISA, WB | Mouse Fab |
TAB-104CT-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-104CT-F(E)) | FC | Mouse Fab |
TAB-105CT-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (TAB-105CT-F(E)) | FC, ELISA, WB, Inhib | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-550CQ | Mouse Anti-CSPG4 Recombinant Antibody (clone 9.2.27) | EM, Block, IP, FC, ICC, IF | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0840 | Hi-Affi™ Rabbit Anti-CSPG4 Recombinant Antibody (clone DS840AB) | WB, IHC, FC | Rabbit IgG |
MOR-0105-FY | Rabbit Anti-CSPG4 Recombinant Antibody (clone AFY0076) | ICC, IF, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0070-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0070-LSX) | ELISA | Mouse IgG1 |
HPAB-0071-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0071-LSX) | ELISA | Mouse IgG1 |
HPAB-0072-LSX | Mouse Anti-CSPG4 Recombinant Antibody (HPAB-0072-LSX) | ELISA | Mouse IgG1 |
HPAB-0074-LSX | Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC12) | ELISA, WB | Human IgG |
HPAB-0075-LSX | Human Anti-CSPG4 Recombinant Antibody (HPAB-0075-LSX) | ELISA | Chimeric (mouse/human) IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0073-LSX-S(P) | Human Anti-CSPG4 Recombinant Antibody (clone D2A-1H10-UC8); scFv Fragment | ELISA, WB | Human scFv |
HPAB-1039WJ-S(P) | Human Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-1039WJ-S(P)) | ELISA | Human scFv |
HPAB-1040WJ-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-1040WJ-S(P)) | ELISA | Mouse scFv |
HPAB-1041WJ-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-1041WJ-S(P)) | ELISA | Mouse scFv |
HPAB-1042WJ-S(P) | Mouse Anti-CSPG4 Recombinant Antibody; scFv Fragment (HPAB-1042WJ-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-595-FY-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-595-FY-F(E)) | ELISA, FC | Chimeric (mouse/human) Fab |
HPAB-1039WJ-F(E) | Human Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1039WJ-F(E)) | ELISA | Human Fab |
HPAB-1040WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1040WJ-F(E)) | ELISA | Mouse Fab |
HPAB-1041WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1041WJ-F(E)) | ELISA | Mouse Fab |
HPAB-1042WJ-F(E) | Mouse Anti-CSPG4 Recombinant Antibody; Fab Fragment (HPAB-1042WJ-F(E)) | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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