Human Anti-CXCR2 Recombinant Antibody (clone HY29); Fab Fragment (CAT#: HPAB-0306-YC-F(E))
An isolated antibody HY29 Fab fragment specifically binds to CXC chemokine receptor-2 (CXCR2), wherein the binding molecule can inhibit binding to interleukin 8 (IL-8). Mapping of the N terminus using linear peptides and alanine scanning of CLIPS peptides derived from the N terminus and ECL3 showed that the minimal epitope for the antibody binding was centered around the 31 PFLLD 35 with the second leucine and the aspartic acid key to the binding interaction.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane, microtubules & mitotic spindle.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31999/1855_E6_15_cr5ad88637398d2_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane, microtubules & mitotic spindle.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31999/1855_E6_15_cr5ad88637398d2_selected.jpg
Figure 2 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/1855_F6_25_cr5ad88701bb05e_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/1855_F6_25_cr5ad88701bb05e_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_5.jpg
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_A_8_8.jpg
Figure 5 High expression in spleen
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_4.jpg
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_4.jpg
Figure 6 RNA cell line category: Group enriched (BEWO, HL-60, HMC-1, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180871-CXCR2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180871-CXCR2
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized Fab
- Specificity
- Human CXCR2
- Species Reactivity
- Human, Monkey, Mouse
- Clone
- HY29
- Applications
- ELISA, FC, FuncS
- Related Disease
- Cancer, Inflammatory disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CXCR2; chemokine (C-X-C motif) receptor 2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b; C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; IL-8R B; GRO/MGSA receptor; IL-8 receptor type 2; interleukin 8 receptor B; chemokine (CXC) receptor 2; interleukin 8 receptor; beta; interleukin 8 receptor type 2; interleukin-8 receptor type B; CXCR2 gene for IL8 receptor type B; high affinity interleukin-8 receptor B
- Gene ID
- 3579
- UniProt ID
- P25025
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-050 | Recombinant Anti-Human CXCR2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-002MZ-VHH | Anti-Human CXCR2 Recombinant Antibody (TAB-002MZ-VHH) | FC, Inhib | Single domain antibody |
| TAB-004MZ-VHH | Anti-Human CXCR2 Recombinant Antibody (TAB-004MZ-VHH) | ELISA, IHC, FuncS, SPR | Single domain antibody |
Customer Reviews and Q&As
Customer Review
Q&As
-
Adaptable to Protocol Variations
This antibody is responsive to a variety of methods and experimental circumstances, allowing for greater experiment design freedom. It's really versatile! -
Well-Packaged
The antibody arrived safely packaged, with all essential information and no product damage. The condition upon arrival was superb. -
Confirmatory Band Patterns
The distinct band patterns seen in Western blots with this antibody are confirming and consistent. It certainly simplifies the interpretation of results.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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