Human Anti-CXCR2 scFv-Fc Chimera (VS-0625-FY7) (CAT#: VS-0625-FY7)
Anti-CXCR2 scFv-FC is a recombinant fusion protein designed to specifically target the chemokine receptor CXCR2. This construct leverages a high-affinity single-chain variable fragment (scFv) that recognizes CXCR2, fused to the Fc domain of a human immunoglobulin, typically IgG1. CXCR2 is a crucial G protein-coupled receptor primarily expressed on neutrophils, but also on monocytes, macrophages, and endothelial cells. It plays a pivotal role in mediating immune cell migration, particularly neutrophil recruitment to sites of inflammation and infection, as well as influencing processes like angiogenesis and tumor progression. The scFv-Fc format provides improved stability and extended half-life, and potentially enables Fc-mediated effector functions. This product serves as a valuable tool for research into inflammatory diseases, autoimmune disorders, and cancer, where CXCR2 signaling is a key mediator of pathogenesis and a promising therapeutic target.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane, microtubules & mitotic spindle.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31999/1855_E6_15_cr5ad88637398d2_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane, microtubules & mitotic spindle.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31999/1855_E6_15_cr5ad88637398d2_selected.jpg
Figure 2 IF staining of human cell line THP-1
(Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/1855_F6_25_cr5ad88701bb05e_selected.jpg
(Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/1855_F6_25_cr5ad88701bb05e_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_5.jpg
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_A_8_8.jpg
Figure 5 High expression in spleen
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_4.jpg
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/32017/139603_B_9_4.jpg
Figure 6 RNA cell line category: Group enriched (BEWO, HL-60, HMC-1, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180871-CXCR2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180871-CXCR2
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Specifically binds to human CXCR2. Also binds to macaque monkey CXCR2. Does not bind to human CXCR1, mouse CXCR2, rat CXCR2, or rabbit CXCR2.
- Species Reactivity
- Human, Macaque monkey
- Applications
- ELISA, Flow Cytometry
- Related Disease
- Inflammatory diseases, autoimmune disorders, and cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- C-X-C Motif Chemokine Receptor 2; High Affinity Interleukin-8 Receptor B; Interleukin 8 Receptor, Beta; IL-8 Receptor Type 2; GRO/MGSA Receptor; IL-8R B; CDw128b; CXC-R2; CXCR-2; IL8RB; Chemokine (C-X-C Motif) Receptor 2
- Gene ID
- 3579
- UniProt ID
- P25025
- Long Name
- C-X-C Motif Chemokine Receptor 2
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated upon ligand binding; which is required for desensitization
(Microbial infection) Proteolytically cleaved by Staphylococcus aureus staphopain A/SspP.
This cleavage inhibits CXCR2-dependent neutrophil activation and chemotaxis.
Glycosylation at Asn22
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen
- Protein Refseq
- XP_005246587.1; XP_016859480.1; XP_047300143.1; XP_047300144.1; XP_047300145.1
- Function
- Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor (PubMed:1891716).
Binding of IL-8 to the receptor causes activation of neutrophils.
This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed:8662698).
Binds to IL-8 with high affinity.
Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-050 | Recombinant Anti-Human CXCR2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-002MZ-VHH | Anti-Human CXCR2 Recombinant Antibody (TAB-002MZ-VHH) | FC, Inhib | Single domain antibody |
| TAB-004MZ-VHH | Anti-Human CXCR2 Recombinant Antibody (TAB-004MZ-VHH) | ELISA, IHC, FuncS, SPR | Single domain antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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