Mouse Anti-GPC3 Recombinant Antibody (clone GC33) (CAT#: HPAB-0561-CN)

An anti-glypican 3 antibody GC33 which has strong GPC3 binding activity. GC33 also exhibits particularly strong CDC activity.


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WB

Figure 1 Anti-GPC3 Antibody (HPAB-0561-CN) in WB

Figure 1 Anti-GPC3 Antibody (HPAB-0561-CN) in WB

Western blot analysis was performed with 0.05 µg (lane 1) and 0/1 µg (lane 2) recombinant human GPC3 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with GPC3 Antibody (HPAB-0561-CN) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.

ELISA

Figure 2 Anti-GPC3 Antibody (HPAB-0561-CN) in ELISA

Figure 2 Anti-GPC3 Antibody (HPAB-0561-CN) in ELISA

ELISA analysis of HPAB-0561-CN was performed by coating with recombinant human GPC3 antigen. Then blocked with BSA, and incubated with GPC3 Antibody (HPAB-0561-CN) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Immunogen
  • A fusion protein containing a peptide from 524 Ala to 563 Lys of GPC3 and GST
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human GPC3
  • Species Reactivity
  • Human
  • Clone
  • GC33
  • Applications
  • ELISA
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Glypican 3; Intestinal Protein OCI-5; Glypican Proteoglycan 3; GTR2-2; MXR7; Heparan Sulphate Proteoglycan; Secreted Glypican-3;
    OCI-5; SGBS1; DGSX; SGBS; SDYS; OCI5; SGB

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NABG-069 Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody WB, ELISA, IHC, FuncS Llama VHH
TAB-416CQ Anti-Human GPC3 Therapeutic Single Domain Antibody (HN3) IP, IHC, FuncS, WB, ELISA, Inhib Single domain antibody

Immunotoxin

CAT Product Name Application Type
AGTO-G035E Anti-Gpc3 immunotoxin (scFv)-PE Cytotoxicity assay, Function study

Chimeric Antibody

CAT Product Name Application Type
TAB-157CT Human Anti-GPC3 Recombinant Antibody (TAB-157CT) ELISA, FC, IHC, WB Chimeric (mouse/human) IgG1
TAB-158CT Human Anti-GPC3 Recombinant Antibody (TAB-158CT) ELISA, FC, IHC, WB Chimeric (mouse/human) IgG1
TAB-157CT-S(P) Mouse Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-157CT-S(P)) ELISA, FC, IHC, WB Mouse scFv
TAB-158CT-S(P) Mouse Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-158CT-S(P)) ELISA, FC, IHC, WB Mouse scFv
TAB-157CT-F(E) Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-157CT-F(E)) ELISA, FC, IHC, WB Chimeric (mouse/human) Fab

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1486 Hi-Affi™ Rabbit Anti-GPC3 Recombinant Antibody (clone DS1486AB) IHC-P Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
HPAB-0553-CN Human Anti-GPC3 Recombinant Antibody (clone GES1) ELISA, FC, IF, IP Chimeric (chicken/human) IgG1
HPAB-0554-CN Human Anti-GPC3 Recombinant Antibody (clone huG5S1) ELISA, FC, IF, IP Humanized IgG1
HPAB-0556-CN Human Anti-GPC3 Recombinant Antibody (HPAB-0556-CN) ELISA, WB Chimeric (mouse/human) IgG1, κ
HPAB-0557-CN Human Anti-GPC3 Recombinant Antibody (HPAB-0557-CN) ELISA, WB Chimeric (mouse/human) IgG1, κ
HPAB-0558-CN Mouse Anti-GPC3 Recombinant Antibody (HPAB-0558-CN) ELISA, WB Mouse IgG1

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H14 Afuco™ Anti-GPC3 ADCC Recombinant Antibody (Codrituzumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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