Recombinant Human Anti-H1N1 HA Antibody (UCA)
CAT#: PABZ-184
Recombinant Human Antibody (UCA) is capable of binding to H1N1 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H1N1 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H1N1 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The UCA antibody binds too weakly for cocrystallization with HA head.
Published Data
Figure 2 Summary of analysis of surface plasmon resonance of Fab binding to immobilized HA (A/Solomon Islands/03/2006) head
Schmidt, A. G., Xu, H., Khan, A. R., O’Donnell, T., Khurana, S., King, L. R., ... & Settembre, E. C. (2013). Preconfiguration of the antigen-binding site during affinity maturation of a broadly neutralizing influenza virus antibody. Proceedings of the National Academy of Sciences, 110(1), 264-269.
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Specifications
- Immunogen
- IAV Hemagglutinin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native H1N1 HA
- Species Reactivity
- IAV
- Clone
- UCA
- Applications
- WB 1:1000-1:5000
ELISA Assay-Dependent
Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody UCA has been reported in applications of WB, ELISA, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- HA; Hemagglutinin
- UniProt ID
- A7UPX0
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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