Recombinant Human Anti-H1N1 HA Antibody (UCA) (CAT#: PABZ-184)

Recombinant Human Antibody (UCA) is capable of binding to H1N1 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H1N1 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H1N1 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The UCA antibody binds too weakly for cocrystallization with HA head.

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Figure 1 HA binding KD(10⁻⁶ M) and neutralization IC50 (mg/mL)

Figure 1 HA binding KD(10⁻⁶ M) and neutralization IC50 (mg/mL)

Raymond, D. D., Stewart, S. M., Lee, J., Ferdman, J., Bajic, G., Do, K. T., ... & Del Giudice, G. (2016). Influenza immunization elicits antibodies specific for an egg-adapted vaccine strain. Nature medicine, 22(12), 1465.

Figure 2 Summary of analysis of surface plasmon resonance of Fab binding to immobilized HA (A/Solomon Islands/03/2006) head

Figure 2 Summary of analysis of surface plasmon resonance of Fab binding to immobilized HA (A/Solomon Islands/03/2006) head

Schmidt, A. G., Xu, H., Khan, A. R., O’Donnell, T., Khurana, S., King, L. R., ... & Settembre, E. C. (2013). Preconfiguration of the antigen-binding site during affinity maturation of a broadly neutralizing influenza virus antibody. Proceedings of the National Academy of Sciences, 110(1), 264-269.


Specifications

  • Immunogen
  • IAV Hemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native H1N1 HA
  • Species Reactivity
  • IAV
  • Clone
  • UCA
  • Applications
  • WB 1:1000-1:5000
    ELISA Assay-Dependent
    Neut Assay-Dependent

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody UCA has been reported in applications of WB, ELISA, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • HA; Hemagglutinin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone UCA"

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Humanized Antibody

CAT Product Name Application Type
PABX-070-S (P) Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) WB, ELISA, Neut, FuncS scFv

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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